TY - JOUR
T1 - Expression and contribution of satellite glial cells purinoceptors to pain transmission in sensory ganglia
T2 - An update
AU - Villa, Giovanni
AU - Fumagalli, Marta
AU - Verderio, Claudia
AU - Abbracchio, Maria P.
AU - Ceruti, Stefania
PY - 2010/2
Y1 - 2010/2
N2 - The role of adenosine-5-triphosphate (ATP) and of the ligand-gated P2X 3 receptor in neuronal dorsal root ganglia (DRG) pain transmission is relatively well established. Much less is known about the purinergic system in trigeminal ganglia (TG), which are involved in certain types of untreatable neuropathic and inflammatory pain, as well as in migraine. Emerging data suggest that purinergic metabotropic P2Y receptors on both neurons and satellite glial cells (SGCs) may also participate in both physiological and pathological pain development. Here, we provide an updated literature review on the role of purinergic signaling in sensory ganglia, with special emphasis on P2Y receptors on SGCs. We also provide new original data showing a time-dependent downregulation of P2Y2 and P2Y4 receptor expression and function in purified SGCs cultures from TG, in comparison with primary mixed neuron-SGCs cultures. These data highlight the importance of the neuron-glia cross-talk in determining the SGCs phenotype. Finally, we show that, in mixed TG cultures, both adenine and guanosine induce intracellular calcium transients in neurons but not in SGCs, suggesting that also these purinergic-related molecules can participate in pain signaling. These findings may have relevant implications for the development of new therapeutic strategies for chronic pain treatment.
AB - The role of adenosine-5-triphosphate (ATP) and of the ligand-gated P2X 3 receptor in neuronal dorsal root ganglia (DRG) pain transmission is relatively well established. Much less is known about the purinergic system in trigeminal ganglia (TG), which are involved in certain types of untreatable neuropathic and inflammatory pain, as well as in migraine. Emerging data suggest that purinergic metabotropic P2Y receptors on both neurons and satellite glial cells (SGCs) may also participate in both physiological and pathological pain development. Here, we provide an updated literature review on the role of purinergic signaling in sensory ganglia, with special emphasis on P2Y receptors on SGCs. We also provide new original data showing a time-dependent downregulation of P2Y2 and P2Y4 receptor expression and function in purified SGCs cultures from TG, in comparison with primary mixed neuron-SGCs cultures. These data highlight the importance of the neuron-glia cross-talk in determining the SGCs phenotype. Finally, we show that, in mixed TG cultures, both adenine and guanosine induce intracellular calcium transients in neurons but not in SGCs, suggesting that also these purinergic-related molecules can participate in pain signaling. These findings may have relevant implications for the development of new therapeutic strategies for chronic pain treatment.
KW - cell-to-cell communication
KW - Extracellular nucleotides
KW - nociception
KW - purines
KW - trigeminal ganglia
UR - http://www.scopus.com/inward/record.url?scp=77957373612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957373612&partnerID=8YFLogxK
U2 - 10.1017/S1740925X10000086
DO - 10.1017/S1740925X10000086
M3 - Article
C2 - 20604978
AN - SCOPUS:77957373612
VL - 6
SP - 31
EP - 42
JO - Neuron Glia Biology
JF - Neuron Glia Biology
SN - 1740-925X
IS - 1
ER -