Expression and distribution of heme oxygenase-1 and -2 in rat brown adipose tissue: The modulatory role of the noradrenergic system

Antonio Giordano, Enzo Nisoli, Cristina Tonello, Raffaella Cancello, Michele O. Carruba, Saverio Cinti

Research output: Contribution to journalArticle

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Abstract

To investigate whether brown adipose tissue (BAT) expresses the inducible (HO-1) and the constitutive (HO-2) isoform of heme oxygenase, reverse transcriptase-polymerase chain reaction, Western blotting and immunohistochemistry were performed on interscapular BAT (IBAT) from rats acclimated at environmental temperature or exposed to cold. Both HO isoforms were detected in rat IBAT. They were immunolocalized in the cytoplasm and/or nuclei of brown adipocytes, in parenchymal capillaries, arteries and in some veins and nerves. Whereas cold exposure did not affect HO-2 expression, it significantly increased the expression of HO-1, both at mRNA (about 3-fold) and protein (about 2-fold) levels, reflecting the increased expression of HO-1 in the brown adipocytes and endothelial cells of parenchymal capillaries. Western blotting of cytosolic and nuclear protein extracts from cultured differentiated brown adipocytes showed that HO-1 and HO-2 are indeed localized in the cytosol and nuclei of brown adipocytes, and that noradrenaline stimulation significantly increased their amount in cytosol but not in the nuclear fraction.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalFEBS Letters
Volume487
Issue number2
DOIs
Publication statusPublished - Dec 29 2000

Fingerprint

Brown Adipocytes
Heme Oxygenase-1
Brown Adipose Tissue
Rats
Protein Isoforms
Tissue
Heme Oxygenase (Decyclizing)
Polymerase chain reaction
RNA-Directed DNA Polymerase
Endothelial cells
Nuclear Proteins
Cytosol
Norepinephrine
Western Blotting
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Veins
Cytoplasm
Proteins
Endothelial Cells

Keywords

  • Brown adipose tissue
  • Heat shock protein 32
  • Heme oxygenase
  • Noradrenaline
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression and distribution of heme oxygenase-1 and -2 in rat brown adipose tissue : The modulatory role of the noradrenergic system. / Giordano, Antonio; Nisoli, Enzo; Tonello, Cristina; Cancello, Raffaella; Carruba, Michele O.; Cinti, Saverio.

In: FEBS Letters, Vol. 487, No. 2, 29.12.2000, p. 171-175.

Research output: Contribution to journalArticle

Giordano, Antonio ; Nisoli, Enzo ; Tonello, Cristina ; Cancello, Raffaella ; Carruba, Michele O. ; Cinti, Saverio. / Expression and distribution of heme oxygenase-1 and -2 in rat brown adipose tissue : The modulatory role of the noradrenergic system. In: FEBS Letters. 2000 ; Vol. 487, No. 2. pp. 171-175.
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