Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells

S. Bernardini, S. Cianfarani, A. Spagnoli, M. Annicchiarico-Petruzzelli, G. Melino, R. Massoud, B. Boscherini, A. Finazzi-Agro, R. G. Rosenfeld, G. Federici

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32% and 24% of control, respectively) and 72h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.

Original languageEnglish
Pages (from-to)409-413
Number of pages5
JournalJournal of Neuroendocrinology
Volume6
Issue number4
DOIs
Publication statusPublished - 1994

Fingerprint

Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Protein 2
Tretinoin
Neuroblastoma
Carrier Proteins
Down-Regulation
Somatomedins
Insulin-Like Growth Factor I
Insulin-Like Growth Factor II
Conditioned Culture Medium
Immunoprecipitation
Cell Differentiation
Western Blotting
Ligands
Cell Line
Growth

Keywords

  • Insulin-like growth factor-binding proteins
  • Insulin-like growth factors
  • Neuroblastoma
  • Retinoic acid

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells. / Bernardini, S.; Cianfarani, S.; Spagnoli, A.; Annicchiarico-Petruzzelli, M.; Melino, G.; Massoud, R.; Boscherini, B.; Finazzi-Agro, A.; Rosenfeld, R. G.; Federici, G.

In: Journal of Neuroendocrinology, Vol. 6, No. 4, 1994, p. 409-413.

Research output: Contribution to journalArticle

Bernardini, S. ; Cianfarani, S. ; Spagnoli, A. ; Annicchiarico-Petruzzelli, M. ; Melino, G. ; Massoud, R. ; Boscherini, B. ; Finazzi-Agro, A. ; Rosenfeld, R. G. ; Federici, G. / Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells. In: Journal of Neuroendocrinology. 1994 ; Vol. 6, No. 4. pp. 409-413.
@article{7c946bb9b6a34b88a7c87ac706787429,
title = "Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells",
abstract = "Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32{\%} and 24{\%} of control, respectively) and 72h (2{\%} and 0{\%} of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45{\%} and 18{\%} of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.",
keywords = "Insulin-like growth factor-binding proteins, Insulin-like growth factors, Neuroblastoma, Retinoic acid",
author = "S. Bernardini and S. Cianfarani and A. Spagnoli and M. Annicchiarico-Petruzzelli and G. Melino and R. Massoud and B. Boscherini and A. Finazzi-Agro and Rosenfeld, {R. G.} and G. Federici",
year = "1994",
doi = "10.1111/j.1365-2826.1994.tb00601.x",
language = "English",
volume = "6",
pages = "409--413",
journal = "Journal of Neuroendocrinology",
issn = "0953-8194",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells

AU - Bernardini, S.

AU - Cianfarani, S.

AU - Spagnoli, A.

AU - Annicchiarico-Petruzzelli, M.

AU - Melino, G.

AU - Massoud, R.

AU - Boscherini, B.

AU - Finazzi-Agro, A.

AU - Rosenfeld, R. G.

AU - Federici, G.

PY - 1994

Y1 - 1994

N2 - Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32% and 24% of control, respectively) and 72h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.

AB - Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32% and 24% of control, respectively) and 72h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.

KW - Insulin-like growth factor-binding proteins

KW - Insulin-like growth factors

KW - Neuroblastoma

KW - Retinoic acid

UR - http://www.scopus.com/inward/record.url?scp=0028168651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028168651&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2826.1994.tb00601.x

DO - 10.1111/j.1365-2826.1994.tb00601.x

M3 - Article

C2 - 7527270

AN - SCOPUS:0028168651

VL - 6

SP - 409

EP - 413

JO - Journal of Neuroendocrinology

JF - Journal of Neuroendocrinology

SN - 0953-8194

IS - 4

ER -