Abstract
Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32% and 24% of control, respectively) and 72h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.
| Original language | English |
|---|---|
| Pages (from-to) | 409-413 |
| Number of pages | 5 |
| Journal | Journal of Neuroendocrinology |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1994 |
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Keywords
- Insulin-like growth factor-binding proteins
- Insulin-like growth factors
- Neuroblastoma
- Retinoic acid
ASJC Scopus subject areas
- Endocrinology
- Neuroscience(all)
Cite this
Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells. / Bernardini, S.; Cianfarani, S.; Spagnoli, A.; Annicchiarico-Petruzzelli, M.; Melino, G.; Massoud, R.; Boscherini, B.; Finazzi-Agro, A.; Rosenfeld, R. G.; Federici, G.
In: Journal of Neuroendocrinology, Vol. 6, No. 4, 1994, p. 409-413.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells
AU - Bernardini, S.
AU - Cianfarani, S.
AU - Spagnoli, A.
AU - Annicchiarico-Petruzzelli, M.
AU - Melino, G.
AU - Massoud, R.
AU - Boscherini, B.
AU - Finazzi-Agro, A.
AU - Rosenfeld, R. G.
AU - Federici, G.
PY - 1994
Y1 - 1994
N2 - Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32% and 24% of control, respectively) and 72h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.
AB - Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37kDa and 25kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48h (32% and 24% of control, respectively) and 72h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37kDa and 25kDa bands after 72h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.
KW - Insulin-like growth factor-binding proteins
KW - Insulin-like growth factors
KW - Neuroblastoma
KW - Retinoic acid
UR - http://www.scopus.com/inward/record.url?scp=0028168651&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028168651&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2826.1994.tb00601.x
DO - 10.1111/j.1365-2826.1994.tb00601.x
M3 - Article
C2 - 7527270
AN - SCOPUS:0028168651
VL - 6
SP - 409
EP - 413
JO - Journal of Neuroendocrinology
JF - Journal of Neuroendocrinology
SN - 0953-8194
IS - 4
ER -