Expression and function of IL-12 and IL-18 receptors on human tonsillar B cells

I. Airoldi, G. Gri, J. D. Marshall, A. Corcione, P. Facchetti, R. Guglielmino, G. Trinchieri, V. Pistoia

Research output: Contribution to journalArticle

Abstract

IL-12 activates murine and human B cells, but little information is available as to the expression and function of IL-12R on human B lymphocytes. Here we show flint the latter cells, freshly isolated from human tonsils, expressed the transcripts of both β1 and β2 chains of IL-12R and that β2 chain mRNA was selectively increased (4- to 5-fold) by incubation with Staphylococcus aureus Cowan I bacteria or IL-12. B ceil stimulation with IL-12 induced de novo expression of the transcripts of the two chains of IL-18R, i.e., IL-1 receptor-related protein and accessory protein-like. Functional studies showed that both IL-12 and IL-18 signaled to B cells through the NF-κB pathway. In the case of IL-12, no involvement of STAT transcription factors, and in particular of STAT-4, was detected, c-rel and p50 were identified as the members of NF-κB family involved in IL-12-mediated signal transduction to B cells. IL-12 and IL-18 synergized in the induction of IFN-γ production by tonslilar B cells, but not in the stimulation of B cell differentiation, although either cytokine promoted IgM secretion in culture supernatants. Finally, naive but not germinal center or memory, tonsillar B cells were identified as the exclusive IL-12 targets in terms of induction of NF-κB activation and of IFN-γ production.

Original languageEnglish
Pages (from-to)6880-6888
Number of pages9
JournalJournal of Immunology
Volume165
Issue number12
Publication statusPublished - Dec 15 2000

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Interleukin-18 Receptors
Interleukin-12
B-Lymphocytes
Interleukin-18
STAT Transcription Factors
Germinal Center
Interleukin-1 Receptors
Palatine Tonsil
Interleukin-2
Immunoglobulin M
Staphylococcus aureus
Cell Differentiation
Signal Transduction
Proteins
Cytokines
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Airoldi, I., Gri, G., Marshall, J. D., Corcione, A., Facchetti, P., Guglielmino, R., ... Pistoia, V. (2000). Expression and function of IL-12 and IL-18 receptors on human tonsillar B cells. Journal of Immunology, 165(12), 6880-6888.

Expression and function of IL-12 and IL-18 receptors on human tonsillar B cells. / Airoldi, I.; Gri, G.; Marshall, J. D.; Corcione, A.; Facchetti, P.; Guglielmino, R.; Trinchieri, G.; Pistoia, V.

In: Journal of Immunology, Vol. 165, No. 12, 15.12.2000, p. 6880-6888.

Research output: Contribution to journalArticle

Airoldi, I, Gri, G, Marshall, JD, Corcione, A, Facchetti, P, Guglielmino, R, Trinchieri, G & Pistoia, V 2000, 'Expression and function of IL-12 and IL-18 receptors on human tonsillar B cells', Journal of Immunology, vol. 165, no. 12, pp. 6880-6888.
Airoldi I, Gri G, Marshall JD, Corcione A, Facchetti P, Guglielmino R et al. Expression and function of IL-12 and IL-18 receptors on human tonsillar B cells. Journal of Immunology. 2000 Dec 15;165(12):6880-6888.
Airoldi, I. ; Gri, G. ; Marshall, J. D. ; Corcione, A. ; Facchetti, P. ; Guglielmino, R. ; Trinchieri, G. ; Pistoia, V. / Expression and function of IL-12 and IL-18 receptors on human tonsillar B cells. In: Journal of Immunology. 2000 ; Vol. 165, No. 12. pp. 6880-6888.
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