Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes

R. Zamarchi, P. Allavena, A. Borsetti, L. Stievano, V. Tosello, N. Marcato, G. Esposito, V. Roni, C. Paganin, G. Bianchi, F. Titti, P. Verani, G. Gerosa, A. Amadori

Research output: Contribution to journalArticle

Abstract

In this paper we addressed the expression of the HIV co-receptors CXCR-4 and CCR-5 in human thymocytes by phenotypic, molecular and functional approaches. Cytofluorimetric analysis disclosed that CXCR-4 was constitutively expressed by freshly isolated thymocytes (∼10 000 molecules/cell in about 30% of thymocytes); the receptor was endowed with functional activity, as it mediated polarization, migration and intracellular Ca 2+ increase in response to its ligand, SDF-1. On the contrary, CCR-5 expression in freshly isolated thymocytes was significantly lower (

Original languageEnglish
Pages (from-to)321-330
Number of pages10
JournalClinical and Experimental Immunology
Volume127
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • CCR-5
  • CXCR-4
  • HIV
  • Thymus

ASJC Scopus subject areas

  • Immunology

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    Zamarchi, R., Allavena, P., Borsetti, A., Stievano, L., Tosello, V., Marcato, N., Esposito, G., Roni, V., Paganin, C., Bianchi, G., Titti, F., Verani, P., Gerosa, G., & Amadori, A. (2002). Expression and functional activity of CXCR-4 and CCR-5 chemokine receptors in human thymocytes. Clinical and Experimental Immunology, 127(2), 321-330. https://doi.org/10.1046/j.1365-2249.2002.01775.x