Expression and functional properties of proteins encoded in the x-II ORF of HTLV-I

D. M. D'Agostino, L. Zotti, T. Ferro, I. Cavallori, M. Silic-Benussi, L. Chieco-Bianchi, V. Ciminale

Research output: Contribution to journalArticle

Abstract

With the aim of identifying viral proteins that contribute to the distinctive properties of HTLV-I biology and pathogenicity, several laboratories have investigated the coding potential of the X region of the genome, which includes five partially overlapping open reading frames (ORFs). We and others have shown that, in addition to the essential regulatory proteins Rex and Tax, a number of accessory proteins encoded in the X region can be produced by alternative splicing and multicistronic translation. One X region ORF, termed X-II, produces two protein isoforms named Tof/p30II and p13II, which are expressed from a doubly- and singly-spliced mRNA, respectively. Initial functional analyses demonstrated that Tof/p30II is a nucleolar/nuclear protein that possesses a region capable of binding to RNA, and p13II is a mitochondrial protein that alters the morphology and function of this organelle. Together with data from other laboratories demonstrating the production of antibodies and CTL against x-II ORF products in HTLV-I infected subjects and the requirement of this ORF for efficient viral replication in vivo, these findings suggest that further characterization of Tof/p30II and p13II will yield insight into remaining undefined aspects of HTLV-I pathogenicity and replication.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalVirus Research
Volume78
Issue number1-2
DOIs
Publication statusPublished - 2001

Keywords

  • HTLV-I
  • Mitochondria
  • Nucleolus
  • Nucleus
  • Splicing
  • X region

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

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    D'Agostino, D. M., Zotti, L., Ferro, T., Cavallori, I., Silic-Benussi, M., Chieco-Bianchi, L., & Ciminale, V. (2001). Expression and functional properties of proteins encoded in the x-II ORF of HTLV-I. Virus Research, 78(1-2), 35-43. https://doi.org/10.1016/S0168-1702(01)00282-9