Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells

L. Trentin, R. Zambello, C. Benati, M. Cassatella, C. Agostini, P. Bulian, F. Adami, G. Carra, G. Pizzolo, G. Semenzato

Research output: Contribution to journalArticle

Abstract

Hairy cell leukemia is a chronic lymphoproliferative disorder characterized by the expansion of neoplastic B-cells expressing the p55 chain of the interleukin 2 receptor (IL-2R) system that is recognized by anti-CD25 monoclonal antibodies (mAb) and binds interleukin 2 (IL-2) with low affinity. In the present study we investigated leukemic hairy cells (HC) for the presence of the p75 IL-2R chain which binds IL-2 with intermediate affinity and plays a crucial role in transducing the message to the cell. For this purpose, we tested highly enriched leukemic HC from six hairy cell leukemia patients for the presence of IL-2R transcripts and for the expression of the p55 and p75 IL-2R chains on their surface membrane by flow cytometry and immunoprecipitation analyses. The functional role of IL-2 in the regulation of HC proliferation was also investigated. Our results indicate that freshly isolated HC express detectable messages for both the p75 IL-2R and the p55 IL-2R. Flow cytometry analysis demonstrated detectable levels of p75 IL-2R on the HC from all patients tested. A mixture of two specific mAb was able to immunoprecipitate detectable amounts of p75 IL-2R from leukemic HC. When leukemic HC were cultured in the presence of several concentrations of IL-2 a low proliferative response was observed. Moreover, the IL-2-driven proliferation of HC was markedly inhibited by anti-p75 IL-2R mAb and to a lesser extent by anti-p55 IL-2R mAb. These findings provide direct evidence of the expression of different IL-2 receptors on leukemic HC and suggest that these molecules might play a role in leukemic cell growth.

Original languageEnglish
Pages (from-to)5223-5228
Number of pages6
JournalCancer Research
Volume52
Issue number19
Publication statusPublished - 1992

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Interleukin-2 Receptors
Interleukin-2
Monoclonal Antibodies
Hairy Cell Leukemia
Flow Cytometry
Cell Proliferation
Lymphoproliferative Disorders
Immunoprecipitation
Cultured Cells
B-Lymphocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Trentin, L., Zambello, R., Benati, C., Cassatella, M., Agostini, C., Bulian, P., ... Semenzato, G. (1992). Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells. Cancer Research, 52(19), 5223-5228.

Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells. / Trentin, L.; Zambello, R.; Benati, C.; Cassatella, M.; Agostini, C.; Bulian, P.; Adami, F.; Carra, G.; Pizzolo, G.; Semenzato, G.

In: Cancer Research, Vol. 52, No. 19, 1992, p. 5223-5228.

Research output: Contribution to journalArticle

Trentin, L, Zambello, R, Benati, C, Cassatella, M, Agostini, C, Bulian, P, Adami, F, Carra, G, Pizzolo, G & Semenzato, G 1992, 'Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells', Cancer Research, vol. 52, no. 19, pp. 5223-5228.
Trentin L, Zambello R, Benati C, Cassatella M, Agostini C, Bulian P et al. Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells. Cancer Research. 1992;52(19):5223-5228.
Trentin, L. ; Zambello, R. ; Benati, C. ; Cassatella, M. ; Agostini, C. ; Bulian, P. ; Adami, F. ; Carra, G. ; Pizzolo, G. ; Semenzato, G. / Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells. In: Cancer Research. 1992 ; Vol. 52, No. 19. pp. 5223-5228.
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AU - Benati, C.

AU - Cassatella, M.

AU - Agostini, C.

AU - Bulian, P.

AU - Adami, F.

AU - Carra, G.

AU - Pizzolo, G.

AU - Semenzato, G.

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