Expression and hormonal modulation of the thromboxane A2 receptor gene in mammalian testicular arteries

Maria Matteo, Ettore Cicinelli, Raffaele Luigi Sciorsci, Elvira Grandone, Giuseppe Cardo, Donatella Colaizzo, Annalisa Rizzo, Pantaleo Greco

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Thromboxane A2 (TXA2) is a biologically potent arachidonate metabolite which causes vasoconstriction and platelet aggregation. This study aimed to evaluate the presence of TXA2 receptors in mammalian testicular arteries and the influence of exogenous gonadotropin administration on their expression. Design: Experimental study. Setting: Experimental animals in an academic research environment. Animal(s): Seven rams aged 4-6 years, weighing 60-90 kg. Intervention(s): Both testicular arteries of seven rams were extracted before (from one testis) and after (from the opposite testis) IV administration of 5,000 IU human chorionic gonadotropin (hCG). Main Outcome Measure(s): The expression of the TXA2 receptor gene was investigated by the real-time polymerase chain reaction. Data were analyzed by means of Student t test. Results were expressed as mean ± standard deviation. A P value of 2 messenger RNA receptor in all the basal samples, but the expression of TXA2 receptor gene was undetectable in all the arteries obtained 24 h after the administration of hCG. Conclusion(s): These preliminary results demonstrated for the first time the presence of TXA2 receptors in mammalian testicular arteries. Results also showed that their expression was down-regulated after hCG administration.

Original languageEnglish
Pages (from-to)1276-1280
Number of pages5
JournalFertility and Sterility
Volume85
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Apr 2006

Keywords

  • hCG
  • ram
  • testicular artery
  • Thromboxane A receptor

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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