Expression and localization of the homeodomain-containing protein HEX in human thyroid tumors

Angela V. D'Elia, Gianluca Tell, Diego Russo, Franco Arturi, Fabio Puglisi, Guidalberto Manfioletti, Valter Gattei, David L. Mack, Palmina Cataldi, Sebastiano Filetti, Carla Di Loreto, Giuseppe Damante

Research output: Contribution to journalArticlepeer-review

Abstract

Homeobox genes are involved in neoplastic transformation of both epithelial and hemopoietic tissues. The divergent homeobox gene HEX is expressed in the anterior visceral endoderm during early mouse development and in some adult tissues of endodermal origin, including liver and thyroid. Whereas a role in leukemyogenesis has been proposed already, few data are available on the involvement of HEX in human epithelial tumors. Herein, we analyzed HEX expression and subcellular localization in a series of 55 human thyroid tumors and in several tumoral cell lines. HEX mRNA was detected by RT-PCR either in normal tissues or in thyroid adenomas and differentiated (papillary and follicular) carcinomas. HEX mRNA was also expressed in most undifferentiated carcinomas. Subcellular localization of HEX protein was investigated by immunohistochemistry. In normal tissues and adenomas, HEX protein was present both in nucleus and cytoplasm. In contrast, both differentiated and undifferentiated carcinomas, as well as the tumoral cell lines investigated, showed HEX protein only in the cytoplasm. These findings suggest that regulation of HEX entry in the nucleus of thyrocytes may represent a critical step during human thyroid tumorigenesis.

Original languageEnglish
Pages (from-to)1376-1383
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume87
Issue number3
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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