Expression and modulation of a mononuclear phagocyte differentiation antigen (PAM-1) during in vitro maturation of peripheral blood monocytes

Guido Poli, Ji Ming Wang, Luigi Ruco, Silvano Rossini, Andrea Biondi, Alberto Mantovani, Stefania Uccini

Research output: Contribution to journalArticle

Abstract

Human macrophages obtained by in vitro maturation of peripheral blood monocytes express a surface antigen, PAM-1, recognized by a monoclonal antibody and typical of pulmonary alveolar and tissue macrophages. PAM-1, undetectable in freshly isolated peripheral blood monocytes, was expressed in monocyte-derived macrophages after 3 days of in vitro adherent culture and was maximal after 14-15 days (50%-60% of positive cells). Similar levels of PAM-1 positivity were observed in non-adherent monocyte-derived macrophages suggesting that cell adhesion was not a critical requisite for the expression of this antigen. Bacterial lipolysaccharide and a monocyte chemotactic protein preparation respectively suppressed and upregulated PAM-1 expression in monocyte-derived macrophages. In contrast interferon-γ, although enhancing the levels of class II HLA-DR antigen in monocyte-derived macrophages, did not influence the kinetics of appearance and the levels of PAM-1 in these cells. Thus, expression of PAM-1, which is restricted to certain stages of the monocyte-macrophage differentiation pathway, is also differentially modulated by activation signals, which can be present in the microenvironment of inflammed tissues.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalInternational Journal of Clinical & Laboratory Research
Volume23
Issue number1-4
DOIs
Publication statusPublished - Dec 1993

Keywords

  • Differentiation
  • Lipopolysaccharide
  • Monocyte chemotactic protein
  • Mononuclear phagocyte
  • PAM-1

ASJC Scopus subject areas

  • Clinical Biochemistry

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