Expression and modulation of homeobox genes from cluster B in endothelial cells

Dorina Belotti, Nathalie Clausse, Domenico Flagiello, Younes Alami, Marc Daukandt, Christophe Deroanne, Bernard Malfoy, Edoardo Boncinelli, Antonio Faiella, Vincent Castronovo

Research output: Contribution to journalArticlepeer-review

Abstract

Angiogenesis is a complex phenomenon likely to be under the strict control of a group of transcription factor(s). Homeobox (HOX)-containing proteins have been identified as regulators controlling the coordinated expression of genes involved in organ development and tissue differentiation. In this study, we have demonstrated that human umbilical vein endothelial cells (HUVEC) express 8 of the 10 HOX genes contained in cluster B. Treatment of HUVEC with tissue plasminogen activator (TPA), an agent known to induce morphologic changes in endothelial cells, or vascular endothelium growth factor (VEGF), a proliferative and angiogenesis inducer, results in a specific time-dependent modulation of the eight HOX genes identified. Interestingly, neither basic fibroblast growth factor, an endothelial proliferative agent, nor TNP-470, a fumagillin derivative with potent antiendothelial cell proliferation properties, affected expression of these HOX genes. Specific modulation of HOX genes by differentiating agents but not by proliferative or antiproliferative molecules suggests that they could be involved in the control of the genetic program that coordinates the construction of new blood vessels.

Original languageEnglish
Pages (from-to)1291-1299
Number of pages9
JournalLaboratory Investigation
Volume78
Issue number10
Publication statusPublished - Oct 1998

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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