Expression and modulation of IFN-γ-inducible chemokines (IP-10, Mig, and I-TAC) in human brain endothelium and astrocytes

Possible relevance for the immune invasion of the central nervous system and the pathogenesis of multiple sclerosis

Andrea Salmaggi, Emilio Ciusani, Marco De Rossi, Maurizio Gelati, Anna Dufour, Elena Corsini, Stefano Pagano, Rossana Baccalini, Elisabetta Ferrero, Silvia Scabini, Valerio Silei

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) mediated by blood-derived immune cells invading the CNS. This invasion could be determined by chemokines, and their role within the MS-affected brain is still poorly defined. We investigated the expression by RT-PCR and protein release by ELISA of the interferon-γ (IFN-γ)-inducible chemokines in human brain microvascular endothelial cells (HBMECs) and astrocytes. The monokine induced by IFN-γ (Mig) behaves as a homing chemokine constitutively expressed in HBMECs and astrocytes, whereas the IFN-γ-inducible 10-kDa protein (IP-10) and IFN-inducible T cell alpha-chemoattractant (I-TAC) are induced only after inflammatory stimuli. The biologic activity of IFN-γ-inducible chemokines from an endothelial source was analyzed, and the transendothelial migration of activated lymphocytes was partly antagonized by specific antibodies, especially anti-Mig antibody. Our data highlight the capability of cells of the CNS to activate the chemoattractant machinery in a proinflammatory environment and in MS.

Original languageEnglish
Pages (from-to)631-640
Number of pages10
JournalJournal of Interferon and Cytokine Research
Volume22
Issue number6
DOIs
Publication statusPublished - 2002

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Chemotactic Factors
Chemokines
Astrocytes
Interferons
Multiple Sclerosis
Endothelium
Central Nervous System
T-Lymphocytes
Brain
Endothelial Cells
Chemokine CXCL10
Monokines
Transendothelial and Transepithelial Migration
Demyelinating Diseases
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Polymerase Chain Reaction
Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Cell Biology

Cite this

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title = "Expression and modulation of IFN-γ-inducible chemokines (IP-10, Mig, and I-TAC) in human brain endothelium and astrocytes: Possible relevance for the immune invasion of the central nervous system and the pathogenesis of multiple sclerosis",
abstract = "Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) mediated by blood-derived immune cells invading the CNS. This invasion could be determined by chemokines, and their role within the MS-affected brain is still poorly defined. We investigated the expression by RT-PCR and protein release by ELISA of the interferon-γ (IFN-γ)-inducible chemokines in human brain microvascular endothelial cells (HBMECs) and astrocytes. The monokine induced by IFN-γ (Mig) behaves as a homing chemokine constitutively expressed in HBMECs and astrocytes, whereas the IFN-γ-inducible 10-kDa protein (IP-10) and IFN-inducible T cell alpha-chemoattractant (I-TAC) are induced only after inflammatory stimuli. The biologic activity of IFN-γ-inducible chemokines from an endothelial source was analyzed, and the transendothelial migration of activated lymphocytes was partly antagonized by specific antibodies, especially anti-Mig antibody. Our data highlight the capability of cells of the CNS to activate the chemoattractant machinery in a proinflammatory environment and in MS.",
author = "Andrea Salmaggi and Emilio Ciusani and {De Rossi}, Marco and Maurizio Gelati and Anna Dufour and Elena Corsini and Stefano Pagano and Rossana Baccalini and Elisabetta Ferrero and Silvia Scabini and Valerio Silei",
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T1 - Expression and modulation of IFN-γ-inducible chemokines (IP-10, Mig, and I-TAC) in human brain endothelium and astrocytes

T2 - Possible relevance for the immune invasion of the central nervous system and the pathogenesis of multiple sclerosis

AU - Salmaggi, Andrea

AU - Ciusani, Emilio

AU - De Rossi, Marco

AU - Gelati, Maurizio

AU - Dufour, Anna

AU - Corsini, Elena

AU - Pagano, Stefano

AU - Baccalini, Rossana

AU - Ferrero, Elisabetta

AU - Scabini, Silvia

AU - Silei, Valerio

PY - 2002

Y1 - 2002

N2 - Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) mediated by blood-derived immune cells invading the CNS. This invasion could be determined by chemokines, and their role within the MS-affected brain is still poorly defined. We investigated the expression by RT-PCR and protein release by ELISA of the interferon-γ (IFN-γ)-inducible chemokines in human brain microvascular endothelial cells (HBMECs) and astrocytes. The monokine induced by IFN-γ (Mig) behaves as a homing chemokine constitutively expressed in HBMECs and astrocytes, whereas the IFN-γ-inducible 10-kDa protein (IP-10) and IFN-inducible T cell alpha-chemoattractant (I-TAC) are induced only after inflammatory stimuli. The biologic activity of IFN-γ-inducible chemokines from an endothelial source was analyzed, and the transendothelial migration of activated lymphocytes was partly antagonized by specific antibodies, especially anti-Mig antibody. Our data highlight the capability of cells of the CNS to activate the chemoattractant machinery in a proinflammatory environment and in MS.

AB - Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) mediated by blood-derived immune cells invading the CNS. This invasion could be determined by chemokines, and their role within the MS-affected brain is still poorly defined. We investigated the expression by RT-PCR and protein release by ELISA of the interferon-γ (IFN-γ)-inducible chemokines in human brain microvascular endothelial cells (HBMECs) and astrocytes. The monokine induced by IFN-γ (Mig) behaves as a homing chemokine constitutively expressed in HBMECs and astrocytes, whereas the IFN-γ-inducible 10-kDa protein (IP-10) and IFN-inducible T cell alpha-chemoattractant (I-TAC) are induced only after inflammatory stimuli. The biologic activity of IFN-γ-inducible chemokines from an endothelial source was analyzed, and the transendothelial migration of activated lymphocytes was partly antagonized by specific antibodies, especially anti-Mig antibody. Our data highlight the capability of cells of the CNS to activate the chemoattractant machinery in a proinflammatory environment and in MS.

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