Expression and Role in Apoptosis of the α- and β-Chains of the IFN-γ Receptor on Human Th1 and Th2 Clones

Francesco Novelli, Mario M. D'Elios, Paola Bernabei, Laurence Ozmen, Laura Rigamonti, Fabio Almerigogna, Guido Forni, Gianfranco Del Prete

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Abstract

The mRNA and protein expression of the α- and β-chains of IFN-γR were evaluated on a panel of human Th1 and Th2 clones. When cultured in IL-2-conditioned medium, both types of clones expressed mRNA for the α- and β-chains, and both chains were present in the cytoplasm. Membrane expression of the α-chain was higher on Th2 than on Th1, whereas the β-chain was poorly expressed on both types but increased following IL-2 withdrawal or PHA stimulation. In addition, both types of clones overexpressed MHC class I glycoproteins following IFN-γR triggering by exogenous IFN-γ, although the kinetics was slower in Th1, and this exposure induced mRNA for IRF-1. When their TCR was triggered in the absence of ARC, Th1 only underwent apoptosis. This activation-induced apoptosis was prevented by blocking of the α-chain or by IFN-γ neutralization. Addition of IFN-γ triggered the apoptosis of Th2 clones. Apoptosis of both types of clones was mediated by autocrine or exogenous IFN-γ through the up-regulation of Fas-L expression, since anti-IFN-γRα mAb inhibited its expression on Th1 and exogenous IFN-γ increased its expression on Th2. These results indicate that activated human Th1 and Th2 lymphocytes express IFN-γR α- and β-chains and are both sensitive to signals provided by IFN-γ. Data also suggest that IFN-γ is critical for switching off their responses.

Original languageEnglish
Pages (from-to)206-213
Number of pages8
JournalJournal of Immunology
Volume159
Issue number1
Publication statusPublished - 1997

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Clone Cells
Apoptosis
Messenger RNA
Interleukin-2
AIDS-Related Complex
Conditioned Culture Medium
Glycoproteins
Cytoplasm
Up-Regulation
Lymphocytes
Membranes
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Novelli, F., D'Elios, M. M., Bernabei, P., Ozmen, L., Rigamonti, L., Almerigogna, F., ... Del Prete, G. (1997). Expression and Role in Apoptosis of the α- and β-Chains of the IFN-γ Receptor on Human Th1 and Th2 Clones. Journal of Immunology, 159(1), 206-213.

Expression and Role in Apoptosis of the α- and β-Chains of the IFN-γ Receptor on Human Th1 and Th2 Clones. / Novelli, Francesco; D'Elios, Mario M.; Bernabei, Paola; Ozmen, Laurence; Rigamonti, Laura; Almerigogna, Fabio; Forni, Guido; Del Prete, Gianfranco.

In: Journal of Immunology, Vol. 159, No. 1, 1997, p. 206-213.

Research output: Contribution to journalArticle

Novelli, F, D'Elios, MM, Bernabei, P, Ozmen, L, Rigamonti, L, Almerigogna, F, Forni, G & Del Prete, G 1997, 'Expression and Role in Apoptosis of the α- and β-Chains of the IFN-γ Receptor on Human Th1 and Th2 Clones', Journal of Immunology, vol. 159, no. 1, pp. 206-213.
Novelli, Francesco ; D'Elios, Mario M. ; Bernabei, Paola ; Ozmen, Laurence ; Rigamonti, Laura ; Almerigogna, Fabio ; Forni, Guido ; Del Prete, Gianfranco. / Expression and Role in Apoptosis of the α- and β-Chains of the IFN-γ Receptor on Human Th1 and Th2 Clones. In: Journal of Immunology. 1997 ; Vol. 159, No. 1. pp. 206-213.
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