Expression and synthesis of fibronectin and laminin by an intestinal epithelial cell line

Susanna Scarpa, Gabriella D'orazi, Andrea Modesti, Sandro Valia, Flavia Zucco, Annalaura Stammati

Research output: Contribution to journalArticlepeer-review

Abstract

An intestinal epithelial cell line (IEC-17), undergoing a process of progressive morphological differentiation, was analysed for expression and synthesis of the extracellular matrix glycoproteins, fibronectin (FN) and laminin (LM). FN and LM cell surface expression was detected by immunoelectron microscopy, while intracytoplasmic accumulation was shown by immunofluorescence. 35S-methionine metabolic labelling was also performed to demonstrate FN and LM synthesis by IEC-17. We have compared two different maturation stages of the cell culture and have found that either early epithelial monolayer cells or later multistratified organoid structure cells expressed and produced large amounts of both proteins. These results indicate that FN and LM are constantly present during the process of IEC-17 organoid maturation: we can hypothesize that the two proteins act as mediators of cell to cell and cell to substrate adhesion interactions and, probably, have an active regulatory role in the process of intestinal epithelial cell differentiation.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalTissue and Cell
Volume20
Issue number3
DOIs
Publication statusPublished - 1988

Keywords

  • extracellular matrix
  • fibroncciin
  • Intestinal epithelium
  • laminin

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

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