Expression, crystallization and preliminary crystallographic analysis of the extracellular IgV-like domain of the human natural killer cell inhibitory receptor p75/AIRM1

Nazzareno Dimasi, Lorenzo Moretta, Roberto Biassoni, Roy A. Mariuzza

Research output: Contribution to journalArticle


p75/AIRM1 (Siglec-7) is a sialic acid-binding Ig-like lectin recently identified as an inhibitory receptor on natural killer cells. The expression, in vitro folding, circular-dichroism spectroscopy, crystallization and preliminary X-ray characterization of the Ig-V like domain of p75/AIRM1 are reported. X-ray data were collected from a single crystal at 100 K, with a maximum useful diffraction pattern extending to 1.45 Å resolution on a synchrotron source. The crystal belongs to a primitive monoclinic space group, with unit-cell parameters a = 32.65, b = 49.72, c = 39.79 Å, α = γ = 90, β = 113°. The systematic absences indicate that the space group is P21. Assuming one molecule per asymmetric unit, V M (the Matthews coefficient) was calculated to be 1.879 Å 3Da-1 and the solvent content was estimated to be 32.01%.

Original languageEnglish
Pages (from-to)1856-1858
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Issue number10
Publication statusPublished - Oct 1 2003


ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

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