Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy

Carolina Scagnolari, Carla Selvaggi, Luisa Chiavuzzo, Teresa Carbone, Lorenzo Zaffiri, Gabriella D'Ettorre, Enrico Girardi, Ombretta Turriziani, Vincenzo Vullo, Guido Antonelli

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: The aim of this study was to evaluate whether gene expression of Toll-like receptor (TLR)-3, TLR4, TLR7 and TLR9 was impaired in patients with chronic HIV-1 infection who were failing to respond to antiretroviral therapy. Methods: Transcripts encoding TLRs were assayed by quantitative real time RT-PCR on peripheral blood mononuclear cells derived from patients with HIV-1 infection who were responding or not responding to antiretroviral therapy and healthy control subjects. Results: Chronic HIV-1-infected patients who failed to respond to therapy showed reduced expression of TLRs 3, 4 and 9, together with increased expression of TLR7, as compared to healthy subjects. Moreover, a trend towards a higher expression of TLR3 and TLR9 was observed in responder patients compared with non-responders. In addition, we found lower levels of TRLs 3, 7 and 9 in patients with high levels of HIV-1 RNA compared to those with lower levels of viremia. Conclusions: These findings demonstrate that in chronic HIV-1-positive patients who were failing to respond to the therapy, there were substantial changes in TLRs expression. This is likely to be an important determinant of the clinical course of HIV-1 infection.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalIntervirology
Volume52
Issue number2
DOIs
Publication statusPublished - May 2009

Fingerprint

HIV-1
HIV Infections
Healthy Volunteers
Therapeutics
Toll-Like Receptor 3
Viremia
Real-Time Polymerase Chain Reaction
Blood Cells
RNA
Gene Expression

Keywords

  • Human immunodeficiency virus
  • Pathogenesis
  • Pattern recognition receptors
  • Toll-like receptors
  • Viremia

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Scagnolari, C., Selvaggi, C., Chiavuzzo, L., Carbone, T., Zaffiri, L., D'Ettorre, G., ... Antonelli, G. (2009). Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy. Intervirology, 52(2), 107-114. https://doi.org/10.1159/000218082

Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy. / Scagnolari, Carolina; Selvaggi, Carla; Chiavuzzo, Luisa; Carbone, Teresa; Zaffiri, Lorenzo; D'Ettorre, Gabriella; Girardi, Enrico; Turriziani, Ombretta; Vullo, Vincenzo; Antonelli, Guido.

In: Intervirology, Vol. 52, No. 2, 05.2009, p. 107-114.

Research output: Contribution to journalArticle

Scagnolari, C, Selvaggi, C, Chiavuzzo, L, Carbone, T, Zaffiri, L, D'Ettorre, G, Girardi, E, Turriziani, O, Vullo, V & Antonelli, G 2009, 'Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy', Intervirology, vol. 52, no. 2, pp. 107-114. https://doi.org/10.1159/000218082
Scagnolari, Carolina ; Selvaggi, Carla ; Chiavuzzo, Luisa ; Carbone, Teresa ; Zaffiri, Lorenzo ; D'Ettorre, Gabriella ; Girardi, Enrico ; Turriziani, Ombretta ; Vullo, Vincenzo ; Antonelli, Guido. / Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy. In: Intervirology. 2009 ; Vol. 52, No. 2. pp. 107-114.
@article{6fb404f550904334ab0a01435a3e6e0f,
title = "Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy",
abstract = "Objective: The aim of this study was to evaluate whether gene expression of Toll-like receptor (TLR)-3, TLR4, TLR7 and TLR9 was impaired in patients with chronic HIV-1 infection who were failing to respond to antiretroviral therapy. Methods: Transcripts encoding TLRs were assayed by quantitative real time RT-PCR on peripheral blood mononuclear cells derived from patients with HIV-1 infection who were responding or not responding to antiretroviral therapy and healthy control subjects. Results: Chronic HIV-1-infected patients who failed to respond to therapy showed reduced expression of TLRs 3, 4 and 9, together with increased expression of TLR7, as compared to healthy subjects. Moreover, a trend towards a higher expression of TLR3 and TLR9 was observed in responder patients compared with non-responders. In addition, we found lower levels of TRLs 3, 7 and 9 in patients with high levels of HIV-1 RNA compared to those with lower levels of viremia. Conclusions: These findings demonstrate that in chronic HIV-1-positive patients who were failing to respond to the therapy, there were substantial changes in TLRs expression. This is likely to be an important determinant of the clinical course of HIV-1 infection.",
keywords = "Human immunodeficiency virus, Pathogenesis, Pattern recognition receptors, Toll-like receptors, Viremia",
author = "Carolina Scagnolari and Carla Selvaggi and Luisa Chiavuzzo and Teresa Carbone and Lorenzo Zaffiri and Gabriella D'Ettorre and Enrico Girardi and Ombretta Turriziani and Vincenzo Vullo and Guido Antonelli",
year = "2009",
month = "5",
doi = "10.1159/000218082",
language = "English",
volume = "52",
pages = "107--114",
journal = "Intervirology",
issn = "0300-5526",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Expression levels of TLRs involved in viral recognition in PBMCs from HIV-1-infected patients failing antiretroviral therapy

AU - Scagnolari, Carolina

AU - Selvaggi, Carla

AU - Chiavuzzo, Luisa

AU - Carbone, Teresa

AU - Zaffiri, Lorenzo

AU - D'Ettorre, Gabriella

AU - Girardi, Enrico

AU - Turriziani, Ombretta

AU - Vullo, Vincenzo

AU - Antonelli, Guido

PY - 2009/5

Y1 - 2009/5

N2 - Objective: The aim of this study was to evaluate whether gene expression of Toll-like receptor (TLR)-3, TLR4, TLR7 and TLR9 was impaired in patients with chronic HIV-1 infection who were failing to respond to antiretroviral therapy. Methods: Transcripts encoding TLRs were assayed by quantitative real time RT-PCR on peripheral blood mononuclear cells derived from patients with HIV-1 infection who were responding or not responding to antiretroviral therapy and healthy control subjects. Results: Chronic HIV-1-infected patients who failed to respond to therapy showed reduced expression of TLRs 3, 4 and 9, together with increased expression of TLR7, as compared to healthy subjects. Moreover, a trend towards a higher expression of TLR3 and TLR9 was observed in responder patients compared with non-responders. In addition, we found lower levels of TRLs 3, 7 and 9 in patients with high levels of HIV-1 RNA compared to those with lower levels of viremia. Conclusions: These findings demonstrate that in chronic HIV-1-positive patients who were failing to respond to the therapy, there were substantial changes in TLRs expression. This is likely to be an important determinant of the clinical course of HIV-1 infection.

AB - Objective: The aim of this study was to evaluate whether gene expression of Toll-like receptor (TLR)-3, TLR4, TLR7 and TLR9 was impaired in patients with chronic HIV-1 infection who were failing to respond to antiretroviral therapy. Methods: Transcripts encoding TLRs were assayed by quantitative real time RT-PCR on peripheral blood mononuclear cells derived from patients with HIV-1 infection who were responding or not responding to antiretroviral therapy and healthy control subjects. Results: Chronic HIV-1-infected patients who failed to respond to therapy showed reduced expression of TLRs 3, 4 and 9, together with increased expression of TLR7, as compared to healthy subjects. Moreover, a trend towards a higher expression of TLR3 and TLR9 was observed in responder patients compared with non-responders. In addition, we found lower levels of TRLs 3, 7 and 9 in patients with high levels of HIV-1 RNA compared to those with lower levels of viremia. Conclusions: These findings demonstrate that in chronic HIV-1-positive patients who were failing to respond to the therapy, there were substantial changes in TLRs expression. This is likely to be an important determinant of the clinical course of HIV-1 infection.

KW - Human immunodeficiency virus

KW - Pathogenesis

KW - Pattern recognition receptors

KW - Toll-like receptors

KW - Viremia

UR - http://www.scopus.com/inward/record.url?scp=65549155443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549155443&partnerID=8YFLogxK

U2 - 10.1159/000218082

DO - 10.1159/000218082

M3 - Article

VL - 52

SP - 107

EP - 114

JO - Intervirology

JF - Intervirology

SN - 0300-5526

IS - 2

ER -