Expression of β-galactoside α2,6-sialyltransferase does not alter the susceptibility of human colon cancer cells to NK-mediated cell lysis

Fabio Dall'Olio, Erminia Mariani, Andrea Tarozzi, Alessandra Meneghetti, Mariella Chiricolo, Joseph T Y Lau, Andrea Facchini

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The extent of processing of N-linked oligosaccharides and the sialylation of the target cell membranes has been positively correlated with resistance to lysis mediated by NK cells, but a conclusive evidence has never been reached. Colon cancer tissues express an increased activity of β-galactoside α2,6-sialyltransferase (EC 2.4.99.1, α2,6ST), which catalyzes the addition of sialic acid in α2,6-linkage to Galβ1,4GlcNAc (N-acetyllactosamine) sequences of glycoprotein N-linked chains. The resulting increased level of membrane α2,6-sialylation appears to be related with a more invasive behavior of cancer cells. This phenomenon may depend on a decreased sensitivity of colon cancer cells to NK cells. To obtain conclusive evidence on the role played by sialylation of N-linked chains in determining the target cell susceptibility to NK-mediated lysis, human colon cancer cell lines not expressing sialyltransferases acting on N-linked chains were transfected with a rat α2,6ST cDNA. Stable transfectants expressed different levels of α2,6ST activity, were reactive with the Sambucus nigra lectin, specific for α2,6-linked sialic acid, and, compared with control transfectsnts, showed a remarkable decrease in the number of unsubstituted Galβ1,4GlcNAc terminal sequences. The NK susceptibility of these clones was found to be identical to that of control transfectants, either when unstimulated- or IL-2-stimulated lymphocytes were used as effecters, Neuraminidase treatment of target cells does not result in significant changes to NK susceptibility. Our data demonstrate that sialic acid α2,6-linked to N-linked chains of target cell glycoproteins does not play a major role in recognition of the target by human NK cells.

Original languageEnglish
Pages (from-to)507-513
Number of pages7
JournalGlycobiology
Volume7
Issue number4
DOIs
Publication statusPublished - Jun 1997

Fingerprint

Sialyltransferases
Galactosides
N-Acetylneuraminic Acid
Natural Killer Cells
Colonic Neoplasms
Cells
Glycoproteins
Lymphocytes
Neuraminidase
Cell membranes
Oligosaccharides
Interleukin-2
Rats
Complementary DNA
Tissue
Membranes
Processing
Clone Cells
Cell Membrane
Cell Line

Keywords

  • Colon cancer cells
  • Natural killer cells
  • Sialyltransferases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Expression of β-galactoside α2,6-sialyltransferase does not alter the susceptibility of human colon cancer cells to NK-mediated cell lysis. / Dall'Olio, Fabio; Mariani, Erminia; Tarozzi, Andrea; Meneghetti, Alessandra; Chiricolo, Mariella; Lau, Joseph T Y; Facchini, Andrea.

In: Glycobiology, Vol. 7, No. 4, 06.1997, p. 507-513.

Research output: Contribution to journalArticle

Dall'Olio, Fabio ; Mariani, Erminia ; Tarozzi, Andrea ; Meneghetti, Alessandra ; Chiricolo, Mariella ; Lau, Joseph T Y ; Facchini, Andrea. / Expression of β-galactoside α2,6-sialyltransferase does not alter the susceptibility of human colon cancer cells to NK-mediated cell lysis. In: Glycobiology. 1997 ; Vol. 7, No. 4. pp. 507-513.
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AU - Facchini, Andrea

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AB - The extent of processing of N-linked oligosaccharides and the sialylation of the target cell membranes has been positively correlated with resistance to lysis mediated by NK cells, but a conclusive evidence has never been reached. Colon cancer tissues express an increased activity of β-galactoside α2,6-sialyltransferase (EC 2.4.99.1, α2,6ST), which catalyzes the addition of sialic acid in α2,6-linkage to Galβ1,4GlcNAc (N-acetyllactosamine) sequences of glycoprotein N-linked chains. The resulting increased level of membrane α2,6-sialylation appears to be related with a more invasive behavior of cancer cells. This phenomenon may depend on a decreased sensitivity of colon cancer cells to NK cells. To obtain conclusive evidence on the role played by sialylation of N-linked chains in determining the target cell susceptibility to NK-mediated lysis, human colon cancer cell lines not expressing sialyltransferases acting on N-linked chains were transfected with a rat α2,6ST cDNA. Stable transfectants expressed different levels of α2,6ST activity, were reactive with the Sambucus nigra lectin, specific for α2,6-linked sialic acid, and, compared with control transfectsnts, showed a remarkable decrease in the number of unsubstituted Galβ1,4GlcNAc terminal sequences. The NK susceptibility of these clones was found to be identical to that of control transfectants, either when unstimulated- or IL-2-stimulated lymphocytes were used as effecters, Neuraminidase treatment of target cells does not result in significant changes to NK susceptibility. Our data demonstrate that sialic acid α2,6-linked to N-linked chains of target cell glycoproteins does not play a major role in recognition of the target by human NK cells.

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