Expression of β2m-free HLA class I heavy chains in neuroblastoma cell lines

A. Marozzi, R. Meneveri, G. Bunone, C. De Santis, L. Lopalco, A. Beretta, A. Agresti, A. G. Siccardi, G. Della Valle, E. Ginelli

Research output: Contribution to journalArticle

Abstract

Flow cytometry with the specific monoclonal antibody (MoAb) L31 was used to analyse the expression of HLA class I heavy chains not bound with β2-microglobulin (β2m) by neuroblastoma (NB) cell lines IMR-32 and LA-N-1. The cells, which express barely detectable amounts of β2m-free (E31-positive molecules) and β2m-complexed HLA class 1 antigens (W6.32- and BBM.1-reactive molecules), expressed MHC class I molecules not bound to light chains upon differentiation with either retinoic acid or serum starvation. The expression was not accompanied by an increase of surface heterodimers. Conversely, recombinant interferon-γ (rIFN-γ) treatment led IMR-32 and LA-N-1 cells to almost exclusively express β2m-complexed HLA class I heavy chains. Surface β2m-free MHC class I molecules displayed a molecular mass of ~45 kDa and did not bind exogenously added β2m. No changes in the synthesis of either HLA class I and β2m mRNAs or of L31 proteins were observed in differentiated NB cells, thus suggesting that the surface exposure of unusual HLA class I antigens is regulated post-translationally. These findings indicate that, in addition to activated lymphocytes, the surface expression of β2m-free class I heavy chains is a feature of other cell types, such as NB cells.

Original languageEnglish
Pages (from-to)661-667
Number of pages7
JournalScandinavian Journal of Immunology
Volume37
Issue number6
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Immunology

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    Marozzi, A., Meneveri, R., Bunone, G., De Santis, C., Lopalco, L., Beretta, A., Agresti, A., Siccardi, A. G., Della Valle, G., & Ginelli, E. (1993). Expression of β2m-free HLA class I heavy chains in neuroblastoma cell lines. Scandinavian Journal of Immunology, 37(6), 661-667.