TY - JOUR
T1 - Expression of 19 microRNAs in glioblastoma and comparison with other brain neoplasia of grades I-III
AU - Visani, Michela
AU - De Biase, Dario
AU - Marucci, Gianluca
AU - Cerasoli, Serenella
AU - Nigrisoli, Evandro
AU - Bacchi Reggiani, Maria Letizia
AU - Albani, Fiorenzo
AU - Baruzzi, Agostino
AU - Pession, Annalisa
PY - 2014/3
Y1 - 2014/3
N2 - Several biomarkers have been proposed as useful parameters to better specify the prognosis or to delineate new target therapy strategies for glioblastoma patients. MicroRNAs could represent putative target molecules, considering their role in tumorigenesis, cancer progression and their specific tissue expression. Although several studies have tried to identify microRNA signature for glioblastoma, a microRNA profile is still far from being well-defined.In this work the expression of 19 microRNAs (miR-7, miR-9, miR-9*, miR-10a, miR-10b, miR-17, miR-20a, miR-21, miR-26a, miR-27a, miR-31, miR-34a, miR-101, miR-137, miR-182, miR-221, miR-222, miR-330, miR-519d) was evaluated in sixty formalin-fixed and paraffin-embedded glioblastoma samples using a locked nucleic acid real-time PCR. Moreover, a comparison of miRNA expressions was performed between primary brain neoplasias of different grades (grades IV-I).The analysis of 14 validated miRNA expression in the 60 glioblastomas, using three different non-neoplastic references as controls, revealed a putative miRNA signature: mir-10b and miR-21 were up-regulated, while miR-7, miR-31, miR-101, miR-137, miR-222 and miR-330 were down-regulated in glioblastomas. Comparing miRNA expression between glioblastoma group and gliomas of grades I-III, 3 miRNAs (miR-10b, mir-34a and miR-101) showed different regulation statuses between high-grade and low-grade tumors. miR-10b was up-regulated in high grade and significantly down-regulated in low-grade gliomas, suggesting that could be a candidate for a GBM target therapy.This study provides further data for the identification of a miRNA profile for glioblastoma and suggests that different-grade neoplasia could be characterized by different expression of specific miRNAs.
AB - Several biomarkers have been proposed as useful parameters to better specify the prognosis or to delineate new target therapy strategies for glioblastoma patients. MicroRNAs could represent putative target molecules, considering their role in tumorigenesis, cancer progression and their specific tissue expression. Although several studies have tried to identify microRNA signature for glioblastoma, a microRNA profile is still far from being well-defined.In this work the expression of 19 microRNAs (miR-7, miR-9, miR-9*, miR-10a, miR-10b, miR-17, miR-20a, miR-21, miR-26a, miR-27a, miR-31, miR-34a, miR-101, miR-137, miR-182, miR-221, miR-222, miR-330, miR-519d) was evaluated in sixty formalin-fixed and paraffin-embedded glioblastoma samples using a locked nucleic acid real-time PCR. Moreover, a comparison of miRNA expressions was performed between primary brain neoplasias of different grades (grades IV-I).The analysis of 14 validated miRNA expression in the 60 glioblastomas, using three different non-neoplastic references as controls, revealed a putative miRNA signature: mir-10b and miR-21 were up-regulated, while miR-7, miR-31, miR-101, miR-137, miR-222 and miR-330 were down-regulated in glioblastomas. Comparing miRNA expression between glioblastoma group and gliomas of grades I-III, 3 miRNAs (miR-10b, mir-34a and miR-101) showed different regulation statuses between high-grade and low-grade tumors. miR-10b was up-regulated in high grade and significantly down-regulated in low-grade gliomas, suggesting that could be a candidate for a GBM target therapy.This study provides further data for the identification of a miRNA profile for glioblastoma and suggests that different-grade neoplasia could be characterized by different expression of specific miRNAs.
KW - Brain neoplasia
KW - Glioblastoma
KW - Low-grade brain tumors
KW - MicroRNA
KW - Real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=84894376146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84894376146&partnerID=8YFLogxK
U2 - 10.1016/j.molonc.2013.12.010
DO - 10.1016/j.molonc.2013.12.010
M3 - Article
C2 - 24412053
AN - SCOPUS:84894376146
VL - 8
SP - 417
EP - 430
JO - Molecular Oncology
JF - Molecular Oncology
SN - 1574-7891
IS - 2
ER -