Expression of 2 immunoglobulin isotypes, IgM and IgA, with identical idiotype in the B cell lymphoma 1.29

R. Sitia, A. Rubartelli, U. Hammerling

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Abstract

The 1.29 tumor originated in I strain mice as a B cell lymphoma. In its ascitic form, the cells express Ia and Lyb antigens and carry the Fc receptor. The tumor cells synthesize 2 immunoglobulins, IgM and IgA, which have λ-chains and heavy chain idiotypic determinants in common. Double labeling of viable cells in immunofluorescence demonstrates that a majority of cells have only IgM (μ +), a proportion has only IgA (α +), and a small population (usually less that 5%) expresses both immunoglobulin isotypes (μ + α +). Although the relative proportions of μ +, α +, and μ +α + cells fluctuate quite markedly in the serial passages of the tumor, the 3 different subpopulations are consistently observed. The proportion of cells binding fluorescein-labeled anti-μ and rhodamine-labeled anti-α simultaneously in the cytoplasm is slightly higher (+IgA - 1.29 cells, suggesting differentiation rather than selective growth as the cause of the change. Cloning of 1.29 cells by the limiting dilution technique yielded only cell lines of IgM -IgA + phenotype. Implantation of 1 such cell line in nude mice produced a tumor that retained this phenotype in successive passages.

Original languageEnglish
Pages (from-to)1388-1394
Number of pages7
JournalJournal of Immunology
Volume127
Issue number4
Publication statusPublished - 1981

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Immunoglobulin Isotypes
B-Cell Lymphoma
Immunoglobulin A
Immunoglobulin M
Neoplasms
Serial Passage
Phenotype
Indicator Dilution Techniques
Cell Line
Rhodamines
Fc Receptors
Histocompatibility Antigens Class II
Fluorescein
Nude Mice
Fluorescent Antibody Technique
Immunoglobulins
Organism Cloning
Cell Differentiation
Cytoplasm
Growth

ASJC Scopus subject areas

  • Immunology

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Expression of 2 immunoglobulin isotypes, IgM and IgA, with identical idiotype in the B cell lymphoma 1.29. / Sitia, R.; Rubartelli, A.; Hammerling, U.

In: Journal of Immunology, Vol. 127, No. 4, 1981, p. 1388-1394.

Research output: Contribution to journalArticle

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AB - The 1.29 tumor originated in I strain mice as a B cell lymphoma. In its ascitic form, the cells express Ia and Lyb antigens and carry the Fc receptor. The tumor cells synthesize 2 immunoglobulins, IgM and IgA, which have λ-chains and heavy chain idiotypic determinants in common. Double labeling of viable cells in immunofluorescence demonstrates that a majority of cells have only IgM (μ +), a proportion has only IgA (α +), and a small population (usually less that 5%) expresses both immunoglobulin isotypes (μ + α +). Although the relative proportions of μ +, α +, and μ +α + cells fluctuate quite markedly in the serial passages of the tumor, the 3 different subpopulations are consistently observed. The proportion of cells binding fluorescein-labeled anti-μ and rhodamine-labeled anti-α simultaneously in the cytoplasm is slightly higher (+IgA - 1.29 cells, suggesting differentiation rather than selective growth as the cause of the change. Cloning of 1.29 cells by the limiting dilution technique yielded only cell lines of IgM -IgA + phenotype. Implantation of 1 such cell line in nude mice produced a tumor that retained this phenotype in successive passages.

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