Abstract
The 1.29 tumor originated in I strain mice as a B cell lymphoma. In its ascitic form, the cells express Ia and Lyb antigens and carry the Fc receptor. The tumor cells synthesize 2 immunoglobulins, IgM and IgA, which have λ-chains and heavy chain idiotypic determinants in common. Double labeling of viable cells in immunofluorescence demonstrates that a majority of cells have only IgM (μ +), a proportion has only IgA (α +), and a small population (usually less that 5%) expresses both immunoglobulin isotypes (μ + α +). Although the relative proportions of μ +, α +, and μ +α + cells fluctuate quite markedly in the serial passages of the tumor, the 3 different subpopulations are consistently observed. The proportion of cells binding fluorescein-labeled anti-μ and rhodamine-labeled anti-α simultaneously in the cytoplasm is slightly higher (+IgA - 1.29 cells, suggesting differentiation rather than selective growth as the cause of the change. Cloning of 1.29 cells by the limiting dilution technique yielded only cell lines of IgM -IgA + phenotype. Implantation of 1 such cell line in nude mice produced a tumor that retained this phenotype in successive passages.
| Original language | English |
|---|---|
| Pages (from-to) | 1388-1394 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 127 |
| Issue number | 4 |
| Publication status | Published - 1981 |
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ASJC Scopus subject areas
- Immunology
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Expression of 2 immunoglobulin isotypes, IgM and IgA, with identical idiotype in the B cell lymphoma 1.29. / Sitia, R.; Rubartelli, A.; Hammerling, U.
In: Journal of Immunology, Vol. 127, No. 4, 1981, p. 1388-1394.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Expression of 2 immunoglobulin isotypes, IgM and IgA, with identical idiotype in the B cell lymphoma 1.29
AU - Sitia, R.
AU - Rubartelli, A.
AU - Hammerling, U.
PY - 1981
Y1 - 1981
N2 - The 1.29 tumor originated in I strain mice as a B cell lymphoma. In its ascitic form, the cells express Ia and Lyb antigens and carry the Fc receptor. The tumor cells synthesize 2 immunoglobulins, IgM and IgA, which have λ-chains and heavy chain idiotypic determinants in common. Double labeling of viable cells in immunofluorescence demonstrates that a majority of cells have only IgM (μ +), a proportion has only IgA (α +), and a small population (usually less that 5%) expresses both immunoglobulin isotypes (μ + α +). Although the relative proportions of μ +, α +, and μ +α + cells fluctuate quite markedly in the serial passages of the tumor, the 3 different subpopulations are consistently observed. The proportion of cells binding fluorescein-labeled anti-μ and rhodamine-labeled anti-α simultaneously in the cytoplasm is slightly higher (+IgA - 1.29 cells, suggesting differentiation rather than selective growth as the cause of the change. Cloning of 1.29 cells by the limiting dilution technique yielded only cell lines of IgM -IgA + phenotype. Implantation of 1 such cell line in nude mice produced a tumor that retained this phenotype in successive passages.
AB - The 1.29 tumor originated in I strain mice as a B cell lymphoma. In its ascitic form, the cells express Ia and Lyb antigens and carry the Fc receptor. The tumor cells synthesize 2 immunoglobulins, IgM and IgA, which have λ-chains and heavy chain idiotypic determinants in common. Double labeling of viable cells in immunofluorescence demonstrates that a majority of cells have only IgM (μ +), a proportion has only IgA (α +), and a small population (usually less that 5%) expresses both immunoglobulin isotypes (μ + α +). Although the relative proportions of μ +, α +, and μ +α + cells fluctuate quite markedly in the serial passages of the tumor, the 3 different subpopulations are consistently observed. The proportion of cells binding fluorescein-labeled anti-μ and rhodamine-labeled anti-α simultaneously in the cytoplasm is slightly higher (+IgA - 1.29 cells, suggesting differentiation rather than selective growth as the cause of the change. Cloning of 1.29 cells by the limiting dilution technique yielded only cell lines of IgM -IgA + phenotype. Implantation of 1 such cell line in nude mice produced a tumor that retained this phenotype in successive passages.
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M3 - Article
VL - 127
SP - 1388
EP - 1394
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 4
ER -