Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients

Francesco Giannessi, Francesco Ursino, Bruno Fattori, Maria Anita Giambelluca, Maria Concetta Scavuzzo, Andrea Nacci, Francesco Fornai, Riccardo Ruffoli

Research output: Contribution to journalArticle

Abstract

Background: Several works have reported that nitric oxide and free oxygen radicals are up-regulated in nasal polyposis. This study aimed to assess the distribution of peroxynitrite in nasal polyps from nonatopic patients. Occurrence of peroxynitrite also was analyzed in relation with eosinophil infiltration and epithelial alterations. Material/Methods: Hematoxylin and eosin staining was used for histologic study. Peroxynitrite was assessed by 3-nitrotyrosine immunohistochemistry. Quantitative evaluation was done by measuring the total number of eosinophils, the number of 3-nitrotyrosine -positive eosinophils, and the extension of the various epithelial alterations. Results: Hematoxylin and eosin staining showed that the nasal polyp epithelium is characterized by progressive disruption or squamous metaplasia. In both cases, infiltrating eosinophils were found in the epithelium and lamina propria. The regions featuring epithelial disruption exhibited 3-nitrotyrosine immunostaining in eosinophils and epithelial cells; hematoxylin-and-eosin - stained eosinophils and 3-nitrotyrosine - positive eosinophils showed conspicuous variations in number. Within the regions featuring squamous metaplasia, 3-nitrotyrosine-positive eosinophils were rarely found, and the epithelium exhibited 3-nitrotyrosine only in the superficial cells. In these regions, hematoxylin-eosin - stained eosinophils showed slight variations in number. Conclusions: Peroxynitrite plays a pivotal role in the pathophysiology of nasal polyps. In fact, strong expression of peroxynitrite is associated with epithelial disruption, while poor expression of peroxynitrite is associated with squamous metaplasia. Peroxynitrite could influence afflux of eosinophils in the nasal mucosa; moreover, the total number of eosinophils is not critical in generating alterations of nasal polyp mucosa.

Original languageEnglish
JournalMedical Science Monitor
Volume16
Issue number4
Publication statusPublished - 2010

Fingerprint

Nasal Polyps
Peroxynitrous Acid
Eosinophils
Hematoxylin
Eosine Yellowish-(YS)
Nasal Mucosa
Metaplasia
3-nitrotyrosine
Epithelium
Staining and Labeling
Nose
Free Radicals
Reactive Oxygen Species
Nitric Oxide
Mucous Membrane
Epithelial Cells
Immunohistochemistry

Keywords

  • Immunohistochemistry
  • Nasal polyposis
  • Peroxynitrite

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Giannessi, F., Ursino, F., Fattori, B., Giambelluca, M. A., Scavuzzo, M. C., Nacci, A., ... Ruffoli, R. (2010). Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients. Medical Science Monitor, 16(4).

Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients. / Giannessi, Francesco; Ursino, Francesco; Fattori, Bruno; Giambelluca, Maria Anita; Scavuzzo, Maria Concetta; Nacci, Andrea; Fornai, Francesco; Ruffoli, Riccardo.

In: Medical Science Monitor, Vol. 16, No. 4, 2010.

Research output: Contribution to journalArticle

Giannessi, F, Ursino, F, Fattori, B, Giambelluca, MA, Scavuzzo, MC, Nacci, A, Fornai, F & Ruffoli, R 2010, 'Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients', Medical Science Monitor, vol. 16, no. 4.
Giannessi F, Ursino F, Fattori B, Giambelluca MA, Scavuzzo MC, Nacci A et al. Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients. Medical Science Monitor. 2010;16(4).
Giannessi, Francesco ; Ursino, Francesco ; Fattori, Bruno ; Giambelluca, Maria Anita ; Scavuzzo, Maria Concetta ; Nacci, Andrea ; Fornai, Francesco ; Ruffoli, Riccardo. / Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients. In: Medical Science Monitor. 2010 ; Vol. 16, No. 4.
@article{f720e251734543a187887d32867a0aa7,
title = "Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients",
abstract = "Background: Several works have reported that nitric oxide and free oxygen radicals are up-regulated in nasal polyposis. This study aimed to assess the distribution of peroxynitrite in nasal polyps from nonatopic patients. Occurrence of peroxynitrite also was analyzed in relation with eosinophil infiltration and epithelial alterations. Material/Methods: Hematoxylin and eosin staining was used for histologic study. Peroxynitrite was assessed by 3-nitrotyrosine immunohistochemistry. Quantitative evaluation was done by measuring the total number of eosinophils, the number of 3-nitrotyrosine -positive eosinophils, and the extension of the various epithelial alterations. Results: Hematoxylin and eosin staining showed that the nasal polyp epithelium is characterized by progressive disruption or squamous metaplasia. In both cases, infiltrating eosinophils were found in the epithelium and lamina propria. The regions featuring epithelial disruption exhibited 3-nitrotyrosine immunostaining in eosinophils and epithelial cells; hematoxylin-and-eosin - stained eosinophils and 3-nitrotyrosine - positive eosinophils showed conspicuous variations in number. Within the regions featuring squamous metaplasia, 3-nitrotyrosine-positive eosinophils were rarely found, and the epithelium exhibited 3-nitrotyrosine only in the superficial cells. In these regions, hematoxylin-eosin - stained eosinophils showed slight variations in number. Conclusions: Peroxynitrite plays a pivotal role in the pathophysiology of nasal polyps. In fact, strong expression of peroxynitrite is associated with epithelial disruption, while poor expression of peroxynitrite is associated with squamous metaplasia. Peroxynitrite could influence afflux of eosinophils in the nasal mucosa; moreover, the total number of eosinophils is not critical in generating alterations of nasal polyp mucosa.",
keywords = "Immunohistochemistry, Nasal polyposis, Peroxynitrite",
author = "Francesco Giannessi and Francesco Ursino and Bruno Fattori and Giambelluca, {Maria Anita} and Scavuzzo, {Maria Concetta} and Andrea Nacci and Francesco Fornai and Riccardo Ruffoli",
year = "2010",
language = "English",
volume = "16",
journal = "Medical Science Monitor",
issn = "1234-1010",
publisher = "International Scientific Literature Inc.",
number = "4",

}

TY - JOUR

T1 - Expression of 3-nitrotyrosine, a marker for peroxynitrite, in nasal polyps of nonatopic patients

AU - Giannessi, Francesco

AU - Ursino, Francesco

AU - Fattori, Bruno

AU - Giambelluca, Maria Anita

AU - Scavuzzo, Maria Concetta

AU - Nacci, Andrea

AU - Fornai, Francesco

AU - Ruffoli, Riccardo

PY - 2010

Y1 - 2010

N2 - Background: Several works have reported that nitric oxide and free oxygen radicals are up-regulated in nasal polyposis. This study aimed to assess the distribution of peroxynitrite in nasal polyps from nonatopic patients. Occurrence of peroxynitrite also was analyzed in relation with eosinophil infiltration and epithelial alterations. Material/Methods: Hematoxylin and eosin staining was used for histologic study. Peroxynitrite was assessed by 3-nitrotyrosine immunohistochemistry. Quantitative evaluation was done by measuring the total number of eosinophils, the number of 3-nitrotyrosine -positive eosinophils, and the extension of the various epithelial alterations. Results: Hematoxylin and eosin staining showed that the nasal polyp epithelium is characterized by progressive disruption or squamous metaplasia. In both cases, infiltrating eosinophils were found in the epithelium and lamina propria. The regions featuring epithelial disruption exhibited 3-nitrotyrosine immunostaining in eosinophils and epithelial cells; hematoxylin-and-eosin - stained eosinophils and 3-nitrotyrosine - positive eosinophils showed conspicuous variations in number. Within the regions featuring squamous metaplasia, 3-nitrotyrosine-positive eosinophils were rarely found, and the epithelium exhibited 3-nitrotyrosine only in the superficial cells. In these regions, hematoxylin-eosin - stained eosinophils showed slight variations in number. Conclusions: Peroxynitrite plays a pivotal role in the pathophysiology of nasal polyps. In fact, strong expression of peroxynitrite is associated with epithelial disruption, while poor expression of peroxynitrite is associated with squamous metaplasia. Peroxynitrite could influence afflux of eosinophils in the nasal mucosa; moreover, the total number of eosinophils is not critical in generating alterations of nasal polyp mucosa.

AB - Background: Several works have reported that nitric oxide and free oxygen radicals are up-regulated in nasal polyposis. This study aimed to assess the distribution of peroxynitrite in nasal polyps from nonatopic patients. Occurrence of peroxynitrite also was analyzed in relation with eosinophil infiltration and epithelial alterations. Material/Methods: Hematoxylin and eosin staining was used for histologic study. Peroxynitrite was assessed by 3-nitrotyrosine immunohistochemistry. Quantitative evaluation was done by measuring the total number of eosinophils, the number of 3-nitrotyrosine -positive eosinophils, and the extension of the various epithelial alterations. Results: Hematoxylin and eosin staining showed that the nasal polyp epithelium is characterized by progressive disruption or squamous metaplasia. In both cases, infiltrating eosinophils were found in the epithelium and lamina propria. The regions featuring epithelial disruption exhibited 3-nitrotyrosine immunostaining in eosinophils and epithelial cells; hematoxylin-and-eosin - stained eosinophils and 3-nitrotyrosine - positive eosinophils showed conspicuous variations in number. Within the regions featuring squamous metaplasia, 3-nitrotyrosine-positive eosinophils were rarely found, and the epithelium exhibited 3-nitrotyrosine only in the superficial cells. In these regions, hematoxylin-eosin - stained eosinophils showed slight variations in number. Conclusions: Peroxynitrite plays a pivotal role in the pathophysiology of nasal polyps. In fact, strong expression of peroxynitrite is associated with epithelial disruption, while poor expression of peroxynitrite is associated with squamous metaplasia. Peroxynitrite could influence afflux of eosinophils in the nasal mucosa; moreover, the total number of eosinophils is not critical in generating alterations of nasal polyp mucosa.

KW - Immunohistochemistry

KW - Nasal polyposis

KW - Peroxynitrite

UR - http://www.scopus.com/inward/record.url?scp=77954246283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954246283&partnerID=8YFLogxK

M3 - Article

C2 - 20357715

AN - SCOPUS:77954246283

VL - 16

JO - Medical Science Monitor

JF - Medical Science Monitor

SN - 1234-1010

IS - 4

ER -