Expression of a NOS-III-like protein in human astroglial cell culture

Marco Colasanti, Tiziana Persichini, Cinzia Fabrizi, Elisabetta Cavalieri, Giorgio Venturini, Paolo Ascenzi, Giuliana Maria Lauro, Hisanori Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

Evidence for the presence of a type-III nitric oxide synthase-like protein (NOS-III-like protein) in astroglial cells is reported. The mRNA of a NOS-III-like protein is constitutively expressed in human astrocytoma T67 cells, taken as an astroglial model. The nucleotide sequence of the PCR product (422 bp) shares more than 99% identity with the cDNA (from 1588 to 2009) of the human endothelial nitric oxide synthase (NOS-III). The molecular mass of the astroglial NOS-III-like protein is about 140 kDa, as observed for human NOS-III. Moreover, the astroglial NOS-III-like protein is constitutively tyrosine-phosphorylated and associated with caveolin-1. The astroglial NOS-III-like protein is apparently inactive, as reported for phosphorylated human NOS-III associated with caveolin-1.

Original languageEnglish
Pages (from-to)552-555
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number3
DOIs
Publication statusPublished - Nov 27 1998

Keywords

  • Caveolin-1
  • Human astrocytoma
  • Nitric oxide
  • NOS-III
  • Phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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