Expression of a receptor for IgM by human T cells in vitro

L. Moretta, M. Ferrarini, M. L. Durante, M. C. Mingari

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The capacity of human peripheral blood lymphocytes (PBL) to bind antigen IgG antibody (EA(IgG)) and antigen IgM antibody (EA(IgM)) complexes was investigated using a rosette technique with ox erythrocytes coated with rabbit IgG or IgM antibody. It was found that while EA(IgG) rosette forming cells (RFC) were detected on PBL freshly drawn from normal individuals, EA(IgM) RFC were present only in suspensions kept in culture for 24 hr in media supplemented with sera containing very low or no amounts of IgM. Experiments of simultaneous detection of EA(IgG) RFC or EA(IgM) RFC and other membrane markers for human T or B cells together with experiments on purified T or B cell populations indicated that EA(IgG) RFC were formed by both T and B cells, while T cells only were capable of EA(IgM) rosette formation. The specificity of the receptors for IgG and IgM was determined by studying the inhibitory capacity of purified human IgM and IgG in the rosette assay. The receptor for IgG was inhibited by IgG and not by IgM, while the reverse was true for the receptor for IgM.

Original languageEnglish
Pages (from-to)565-569
Number of pages5
JournalEuropean Journal of Immunology
Issue number8
Publication statusPublished - 1975

ASJC Scopus subject areas

  • Immunology


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