Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate

Giovanni Raugei, Alessandra Modesti, Francesca Magherini, Riccardo Marzocchini, Manuela Vecchi, Giampietro Ramponi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalBiotechnology and Applied Biochemistry
Volume23
Issue number3
Publication statusPublished - 1996

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Yeast
Fermentation
Saccharomyces cerevisiae
Ethanol
DNA sequences
Glucose
Muscle
Yeasts
Muscles
acylphosphatase
Experiments
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Raugei, G., Modesti, A., Magherini, F., Marzocchini, R., Vecchi, M., & Ramponi, G. (1996). Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate. Biotechnology and Applied Biochemistry, 23(3), 273-278.

Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate. / Raugei, Giovanni; Modesti, Alessandra; Magherini, Francesca; Marzocchini, Riccardo; Vecchi, Manuela; Ramponi, Giampietro.

In: Biotechnology and Applied Biochemistry, Vol. 23, No. 3, 1996, p. 273-278.

Research output: Contribution to journalArticle

Raugei, G, Modesti, A, Magherini, F, Marzocchini, R, Vecchi, M & Ramponi, G 1996, 'Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate', Biotechnology and Applied Biochemistry, vol. 23, no. 3, pp. 273-278.
Raugei, Giovanni ; Modesti, Alessandra ; Magherini, Francesca ; Marzocchini, Riccardo ; Vecchi, Manuela ; Ramponi, Giampietro. / Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate. In: Biotechnology and Applied Biochemistry. 1996 ; Vol. 23, No. 3. pp. 273-278.
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