Abstract
Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.
| Original language | English |
|---|---|
| Pages (from-to) | 273-278 |
| Number of pages | 6 |
| Journal | Biotechnology and Applied Biochemistry |
| Volume | 23 |
| Issue number | 3 |
| Publication status | Published - 1996 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
Cite this
Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate. / Raugei, Giovanni; Modesti, Alessandra; Magherini, Francesca; Marzocchini, Riccardo; Vecchi, Manuela; Ramponi, Giampietro.
In: Biotechnology and Applied Biochemistry, Vol. 23, No. 3, 1996, p. 273-278.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate
AU - Raugei, Giovanni
AU - Modesti, Alessandra
AU - Magherini, Francesca
AU - Marzocchini, Riccardo
AU - Vecchi, Manuela
AU - Ramponi, Giampietro
PY - 1996
Y1 - 1996
N2 - Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.
AB - Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.
UR - http://www.scopus.com/inward/record.url?scp=0029969996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029969996&partnerID=8YFLogxK
M3 - Article
VL - 23
SP - 273
EP - 278
JO - Biotechnology and Applied Biochemistry
JF - Biotechnology and Applied Biochemistry
SN - 0885-4513
IS - 3
ER -