Expression of adenylate kinase 1 in bovine retinal cytosol

Silvia Ravera, Luca Musante, Daniela Calzia, Isabella Panfoli, Maurizio Bruschi, Giovanni Candiano, Isidoro Mario Pepe, Alessandro Morelli

Research output: Contribution to journalArticlepeer-review

Abstract

Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalCurrent Eye Research
Volume32
Issue number3
DOIs
Publication statusPublished - Mar 2007

Keywords

  • Adenylate kinase
  • Expression
  • Retina
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Fingerprint Dive into the research topics of 'Expression of adenylate kinase 1 in bovine retinal cytosol'. Together they form a unique fingerprint.

Cite this