Expression of adenylate kinase 1 in bovine retinal cytosol

Silvia Ravera, Luca Musante, Daniela Calzia, Isabella Panfoli, Maurizio Bruschi, Giovanni Candiano, Isidoro Mario Pepe, Alessandro Morelli

Research output: Contribution to journalArticlepeer-review


Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalCurrent Eye Research
Issue number3
Publication statusPublished - Mar 2007


  • Adenylate kinase
  • Expression
  • Retina
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems


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