Abstract
Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.
Original language | English |
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Pages (from-to) | 249-257 |
Number of pages | 9 |
Journal | Current Eye Research |
Volume | 32 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2007 |
Keywords
- Adenylate kinase
- Expression
- Retina
- Two-dimensional electrophoresis
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems