Expression of adenylate kinase 1 in bovine retinal cytosol

Silvia Ravera; Luca Musante; Daniela Calzia; Isabella Panfoli; Maurizio Bruschi; Giovanni Candiano; Isidoro Mario Pepe; Alessandro Morelli

doi:10.1080/02713680601161212

Expression of adenylate kinase 1 in bovine retinal cytosol

Silvia Ravera, Luca Musante, Daniela Calzia, Isabella Panfoli, Maurizio Bruschi, Giovanni Candiano, Isidoro Mario Pepe, Alessandro Morelli

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

Original language	English
Pages (from-to)	249-257
Number of pages	9
Journal	Current Eye Research
Volume	32
Issue number	3
DOIs	https://doi.org/10.1080/02713680601161212
Publication status	Published - Mar 2007

Keywords

Adenylate kinase
Expression
Retina
Two-dimensional electrophoresis

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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10.1080/02713680601161212

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abstract = "Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.",

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T1 - Expression of adenylate kinase 1 in bovine retinal cytosol

AU - Ravera, Silvia

AU - Musante, Luca

AU - Calzia, Daniela

AU - Panfoli, Isabella

AU - Bruschi, Maurizio

AU - Candiano, Giovanni

AU - Pepe, Isidoro Mario

AU - Morelli, Alessandro

PY - 2007/3

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AB - Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

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KW - Expression

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KW - Two-dimensional electrophoresis

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Expression of adenylate kinase 1 in bovine retinal cytosol

Abstract

Keywords

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