Abstract
Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.
| Original language | English |
|---|---|
| Pages (from-to) | 249-257 |
| Number of pages | 9 |
| Journal | Current Eye Research |
| Volume | 32 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2007 |
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Keywords
- Adenylate kinase
- Expression
- Retina
- Two-dimensional electrophoresis
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
Cite this
Expression of adenylate kinase 1 in bovine retinal cytosol. / Ravera, Silvia; Musante, Luca; Calzia, Daniela; Panfoli, Isabella; Bruschi, Maurizio; Candiano, Giovanni; Pepe, Isidoro Mario; Morelli, Alessandro.
In: Current Eye Research, Vol. 32, No. 3, 03.2007, p. 249-257.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of adenylate kinase 1 in bovine retinal cytosol
AU - Ravera, Silvia
AU - Musante, Luca
AU - Calzia, Daniela
AU - Panfoli, Isabella
AU - Bruschi, Maurizio
AU - Candiano, Giovanni
AU - Pepe, Isidoro Mario
AU - Morelli, Alessandro
PY - 2007/3
Y1 - 2007/3
N2 - Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.
AB - Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.
KW - Adenylate kinase
KW - Expression
KW - Retina
KW - Two-dimensional electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=33947326274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947326274&partnerID=8YFLogxK
U2 - 10.1080/02713680601161212
DO - 10.1080/02713680601161212
M3 - Article
VL - 32
SP - 249
EP - 257
JO - Current Eye Research
JF - Current Eye Research
SN - 0271-3683
IS - 3
ER -