Expression of adenylate kinase 1 in bovine retinal cytosol

Silvia Ravera, Luca Musante, Daniela Calzia, Isabella Panfoli, Maurizio Bruschi, Giovanni Candiano, Isidoro Mario Pepe, Alessandro Morelli

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalCurrent Eye Research
Volume32
Issue number3
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Adenylate Kinase
Cytosol
Protein Isoforms
Retina
Rod Cell Outer Segment
Adenine Nucleotides
Immunoblotting
Energy Metabolism
Vertebrates
Electrophoresis
Mass Spectrometry
Lasers
Homeostasis
Phosphotransferases
Peptides
Antibodies
Enzymes
adenylate kinase 1

Keywords

  • Adenylate kinase
  • Expression
  • Retina
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Ravera, S., Musante, L., Calzia, D., Panfoli, I., Bruschi, M., Candiano, G., ... Morelli, A. (2007). Expression of adenylate kinase 1 in bovine retinal cytosol. Current Eye Research, 32(3), 249-257. https://doi.org/10.1080/02713680601161212

Expression of adenylate kinase 1 in bovine retinal cytosol. / Ravera, Silvia; Musante, Luca; Calzia, Daniela; Panfoli, Isabella; Bruschi, Maurizio; Candiano, Giovanni; Pepe, Isidoro Mario; Morelli, Alessandro.

In: Current Eye Research, Vol. 32, No. 3, 03.2007, p. 249-257.

Research output: Contribution to journalArticle

Ravera, S, Musante, L, Calzia, D, Panfoli, I, Bruschi, M, Candiano, G, Pepe, IM & Morelli, A 2007, 'Expression of adenylate kinase 1 in bovine retinal cytosol', Current Eye Research, vol. 32, no. 3, pp. 249-257. https://doi.org/10.1080/02713680601161212
Ravera, Silvia ; Musante, Luca ; Calzia, Daniela ; Panfoli, Isabella ; Bruschi, Maurizio ; Candiano, Giovanni ; Pepe, Isidoro Mario ; Morelli, Alessandro. / Expression of adenylate kinase 1 in bovine retinal cytosol. In: Current Eye Research. 2007 ; Vol. 32, No. 3. pp. 249-257.
@article{fdee3950ec0147de9a01a5183fa30c29,
title = "Expression of adenylate kinase 1 in bovine retinal cytosol",
abstract = "Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.",
keywords = "Adenylate kinase, Expression, Retina, Two-dimensional electrophoresis",
author = "Silvia Ravera and Luca Musante and Daniela Calzia and Isabella Panfoli and Maurizio Bruschi and Giovanni Candiano and Pepe, {Isidoro Mario} and Alessandro Morelli",
year = "2007",
month = "3",
doi = "10.1080/02713680601161212",
language = "English",
volume = "32",
pages = "249--257",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Expression of adenylate kinase 1 in bovine retinal cytosol

AU - Ravera, Silvia

AU - Musante, Luca

AU - Calzia, Daniela

AU - Panfoli, Isabella

AU - Bruschi, Maurizio

AU - Candiano, Giovanni

AU - Pepe, Isidoro Mario

AU - Morelli, Alessandro

PY - 2007/3

Y1 - 2007/3

N2 - Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

AB - Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.

KW - Adenylate kinase

KW - Expression

KW - Retina

KW - Two-dimensional electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=33947326274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947326274&partnerID=8YFLogxK

U2 - 10.1080/02713680601161212

DO - 10.1080/02713680601161212

M3 - Article

VL - 32

SP - 249

EP - 257

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 3

ER -