Expression of adhesion molecules and chemotactic cytokines in cultured human mesothelial cells

Nives Jonjić, Giuseppe Peri, Sergio Bernasconi, Francesca Luisa Sciacca, Francesco Colotta, PierGiuseppe Pelicci, Luisa Lanfrancone, Alberto Mantovani

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

The mesothelium is a flat epithelial lining of serous cavities that could gate the traffic of molecules and cells between the circulation and these body compartments. The present study was designed to elucidate the capacity of mesothelial cells to express adhesion molecules and chemoattractant cytokines, two fundamental mechanisms of regulation of leukocyte recruitment. Cultured human mesothelial cells express appreciable levels of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1), and these were increased by in vitro exposure to tumor necrosis factor (TNF), interferon γ (IFN-γ), or TNF and IFN-γ. Interleukin 1 (IL-1) was a less consistent stimulus for adhesion molecule expression in vitro. Unlike endothelial cells, used as a reference cell population, resting or stimulated mesothelial cells did not express E-selectin and ICAM-2, as assessed by flow cytometry. Analysis of VCAM-1 mRNA by reverse transcriptase and polymerase chain reaction using appropriate primers revealed that mesothelial cells expressed both the seven- and the six-Ig domain transcripts, with predominance of the longer species. Monocytes bound appreciably to "resting" and, to a greater extent, to stimulated mesothelial cells. Monocytes exposed to IFN-γ and lipopolysaccharide, used as prototypic activation signals, showed increased capacity to bind mesothelial cells. Anti-CD18 monoclonal antibody significantly inhibited binding of monocytes to mesothelial cells, and this blocking effect was amplified by anti-very late antigen 4. Mesothelial cells were able to express the chemotactic cytokines IL-8 and monocyte chemotactic protein 1 at the mRNA and protein levels. These results indicate that mesothelial cells can express a set of adhesion molecules (ICAM-1 and VCAM-1) overlapping with, but distinct from, that expressed in vascular endothelium (ICAM-1, ICAM-2, VCAM-1, E-selectin), and that these are functionally relevant for interacting with mononuclear phagocytes. The regulated expression of adhesion molecules and chemotactic cytokines by mesothelial cells is probably important in inflammatory and immune reactions that involve serous cavities, such as the long-known macrophage appearance and disappearance reactions.

Original languageEnglish
Pages (from-to)1165-1174
Number of pages10
JournalJournal of Experimental Medicine
Volume176
Issue number4
Publication statusPublished - Oct 1 1992

Fingerprint

Chemokines
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Interferons
Monocytes
E-Selectin
Tumor Necrosis Factor-alpha
Integrin alpha4beta1
Messenger RNA
Chemokine CCL2
Chemotactic Factors
Vascular Endothelium
Phagocytes
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-8
Interleukin-1
Lipopolysaccharides
Flow Cytometry
Leukocytes
Epithelium

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of adhesion molecules and chemotactic cytokines in cultured human mesothelial cells. / Jonjić, Nives; Peri, Giuseppe; Bernasconi, Sergio; Sciacca, Francesca Luisa; Colotta, Francesco; Pelicci, PierGiuseppe; Lanfrancone, Luisa; Mantovani, Alberto.

In: Journal of Experimental Medicine, Vol. 176, No. 4, 01.10.1992, p. 1165-1174.

Research output: Contribution to journalArticle

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AU - Bernasconi, Sergio

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AU - Colotta, Francesco

AU - Pelicci, PierGiuseppe

AU - Lanfrancone, Luisa

AU - Mantovani, Alberto

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