Expression of adhesion molecules, platelet-activating factor, and chemokines by Kaposi's sarcoma cells

Francesca L. Sciacca, Michael Stürzl, Federico Bussolino, Marina Sironi, Heinz Brandstetter, Christian Zietz, Dan Zhou, Cristian Matteucci, Giuseppe Peri, Silvano Sozzani, Roberto Benelli, Marco Arese, Adriana Albini, Francesco Colotta, Alberto Mantovani

Research output: Contribution to journalArticlepeer-review

Abstract

The present study was designed to investigate whether cells cultured from Kaposi's sarcoma (KS), a vascular tumor with a prominent leukocyte infiltration, express molecules important for the recruitment and activation of leukocytes. KS cells expressed intercellular adhesion molecule-1, which was augmented by exposure to IL-1β or TNF-α. Unlike endothelial cells, resting or cytokine-activated KS cells did not express appreciable levels of intercellular adhesion molecule-2, vascular cell adhesion molecule-1, and E- selectin on their surface. Weak expression of vascular cell adhesion molecule-1 mRNA was detectable by Northern blot analysis and, most clearly, by PCR analysis. Upon exposure to inflammatory cytokines, KS cells produced the attractant/activating lipid platelet-activating factor. KS cells expressed appreciable levels of the chemotactic cytokines, monocyte chemotactic protein-1 (MCP-1) and IL-8, as determined by Northern blot analysis, immunoassay, or bioassay. Chemokine production was augmented by IL- 1β or TNF-α. MCP-1 expression was also detected in KS lesions by in situ hybridization. The set of molecules identified in the present study is probably important in determining the prominent leukocyte infiltration observed in KS. Tumor-associated leukocytes may amplify autocrine/paracrine circuits that sustain KS proliferation and contribute to recruitment of host vascular cells.

Original languageEnglish
Pages (from-to)4816-4825
Number of pages10
JournalJournal of Immunology
Volume153
Issue number10
Publication statusPublished - Nov 15 1994

ASJC Scopus subject areas

  • Immunology

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