Expression of AMPA-glutamate receptor B subunit in rat hippocampal GABAergic neurons

Claudia Racca, Maria Vincenza Catania, Hannah Monyer, Bert Sakmann

Research output: Contribution to journalArticlepeer-review

Abstract

The physiological properties of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptor (GluR) channels are determined by their subunit composition. In particular, the expression of the GluR-B subunit determines the divalent ion selectivity of the channel. We studied the distribution of GluR-B mRNA and protein in adult rat hippocampal GABAergic neurons combining non-radioactive in situ hybridization with immunocytochemistry. The majority of GABAergic hippocampal neurons were GluR-B mRNA-positive, but overall the levels of GluR-B transcript were lower than in pyramidal and granule cells, which showed the highest hybridization signal. The different GluR-B mRNA expression levels in GABAergic versus principal neurons were also observed at the protein level. There was a paucity of GluR-B/C immunoreactivity in the vast majority of somata of the GABAergic neurons studied, which contrasted with the strong expression of GluR-B/C proteins in the hippocampal principal neurons. Our results demonstrate the general low expression of the GluR-B subunit mRNA and GluR-B/C proteins in GABAergic hippocampal neurons. Considering the dominant role of the GIuR-B subunit in determining the divalent ion permeability of the receptor, it is likely that most GABAergic neurons express AMPA receptor channels with different calcium permeabilities.

Original languageEnglish
Pages (from-to)1580-1590
Number of pages11
JournalEuropean Journal of Neuroscience
Volume8
Issue number8
Publication statusPublished - 1996

Keywords

  • cRNA probes
  • Digoxigenin
  • Glutamic acid decarboxylase
  • Hippocampus
  • Interneurons

ASJC Scopus subject areas

  • Neuroscience(all)

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