Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

Cristina Limatola, Sabrina Di Bartolomeo, Flavia Trettel, Clotilde Lauro, Maria T. Ciotti, Delio Mercanti, Loriana Castellani, Fabrizio Eusebi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalJournal of Neuroimmunology
Volume134
Issue number1-2
DOIs
Publication statusPublished - Jan 2003

Fingerprint

CXC Chemokines
AMPA Receptors
Glutamate Receptors
Chemotaxis
Ligands
Neurons
Interleukin-8B Receptors
Immunoprecipitation
Cell Movement

Keywords

  • Chemokines
  • Chemotaxis
  • Glutamate
  • Rat cerebellar neurons

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis. / Limatola, Cristina; Di Bartolomeo, Sabrina; Trettel, Flavia; Lauro, Clotilde; Ciotti, Maria T.; Mercanti, Delio; Castellani, Loriana; Eusebi, Fabrizio.

In: Journal of Neuroimmunology, Vol. 134, No. 1-2, 01.2003, p. 61-71.

Research output: Contribution to journalArticle

Limatola, Cristina ; Di Bartolomeo, Sabrina ; Trettel, Flavia ; Lauro, Clotilde ; Ciotti, Maria T. ; Mercanti, Delio ; Castellani, Loriana ; Eusebi, Fabrizio. / Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis. In: Journal of Neuroimmunology. 2003 ; Vol. 134, No. 1-2. pp. 61-71.
@article{6112f43afe964086af4ae37f91a1ad20,
title = "Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis",
abstract = "We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.",
keywords = "Chemokines, Chemotaxis, Glutamate, Rat cerebellar neurons",
author = "Cristina Limatola and {Di Bartolomeo}, Sabrina and Flavia Trettel and Clotilde Lauro and Ciotti, {Maria T.} and Delio Mercanti and Loriana Castellani and Fabrizio Eusebi",
year = "2003",
month = "1",
doi = "10.1016/S0165-5728(02)00401-0",
language = "English",
volume = "134",
pages = "61--71",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier Science B.V.",
number = "1-2",

}

TY - JOUR

T1 - Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

AU - Limatola, Cristina

AU - Di Bartolomeo, Sabrina

AU - Trettel, Flavia

AU - Lauro, Clotilde

AU - Ciotti, Maria T.

AU - Mercanti, Delio

AU - Castellani, Loriana

AU - Eusebi, Fabrizio

PY - 2003/1

Y1 - 2003/1

N2 - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

AB - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

KW - Chemokines

KW - Chemotaxis

KW - Glutamate

KW - Rat cerebellar neurons

UR - http://www.scopus.com/inward/record.url?scp=0037219095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037219095&partnerID=8YFLogxK

U2 - 10.1016/S0165-5728(02)00401-0

DO - 10.1016/S0165-5728(02)00401-0

M3 - Article

C2 - 12507773

AN - SCOPUS:0037219095

VL - 134

SP - 61

EP - 71

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -