Abstract
We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.
| Original language | English |
|---|---|
| Pages (from-to) | 61-71 |
| Number of pages | 11 |
| Journal | Journal of Neuroimmunology |
| Volume | 134 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jan 2003 |
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Keywords
- Chemokines
- Chemotaxis
- Glutamate
- Rat cerebellar neurons
ASJC Scopus subject areas
- Immunology
- Clinical Neurology
- Immunology and Allergy
- Neurology
Cite this
Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis. / Limatola, Cristina; Di Bartolomeo, Sabrina; Trettel, Flavia; Lauro, Clotilde; Ciotti, Maria T.; Mercanti, Delio; Castellani, Loriana; Eusebi, Fabrizio.
In: Journal of Neuroimmunology, Vol. 134, No. 1-2, 01.2003, p. 61-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis
AU - Limatola, Cristina
AU - Di Bartolomeo, Sabrina
AU - Trettel, Flavia
AU - Lauro, Clotilde
AU - Ciotti, Maria T.
AU - Mercanti, Delio
AU - Castellani, Loriana
AU - Eusebi, Fabrizio
PY - 2003/1
Y1 - 2003/1
N2 - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.
AB - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.
KW - Chemokines
KW - Chemotaxis
KW - Glutamate
KW - Rat cerebellar neurons
UR - http://www.scopus.com/inward/record.url?scp=0037219095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037219095&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(02)00401-0
DO - 10.1016/S0165-5728(02)00401-0
M3 - Article
C2 - 12507773
AN - SCOPUS:0037219095
VL - 134
SP - 61
EP - 71
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -