Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

Cristina Limatola; Sabrina Di Bartolomeo; Flavia Trettel; Clotilde Lauro; Maria T. Ciotti; Delio Mercanti; Loriana Castellani; Fabrizio Eusebi

doi:10.1016/S0165-5728(02)00401-0

Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

Cristina Limatola, Sabrina Di Bartolomeo, Flavia Trettel, Clotilde Lauro, Maria T. Ciotti, Delio Mercanti, Loriana Castellani, Fabrizio Eusebi

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Abstract

We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

Original language	English
Pages (from-to)	61-71
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	134
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(02)00401-0
Publication status	Published - Jan 2003

Keywords

Chemokines
Chemotaxis
Glutamate
Rat cerebellar neurons

ASJC Scopus subject areas

Immunology
Clinical Neurology
Immunology and Allergy
Neurology

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10.1016/S0165-5728(02)00401-0

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title = "Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis",

abstract = "We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.",

keywords = "Chemokines, Chemotaxis, Glutamate, Rat cerebellar neurons",

author = "Cristina Limatola and {Di Bartolomeo}, Sabrina and Flavia Trettel and Clotilde Lauro and Ciotti, {Maria T.} and Delio Mercanti and Loriana Castellani and Fabrizio Eusebi",

year = "2003",

month = jan,

doi = "10.1016/S0165-5728(02)00401-0",

language = "English",

volume = "134",

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T1 - Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

AU - Limatola, Cristina

AU - Di Bartolomeo, Sabrina

AU - Trettel, Flavia

AU - Lauro, Clotilde

AU - Ciotti, Maria T.

AU - Mercanti, Delio

AU - Castellani, Loriana

AU - Eusebi, Fabrizio

PY - 2003/1

Y1 - 2003/1

N2 - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

AB - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.

KW - Chemokines

KW - Chemotaxis

KW - Glutamate

KW - Rat cerebellar neurons

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DO - 10.1016/S0165-5728(02)00401-0

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AN - SCOPUS:0037219095

VL - 134

SP - 61

EP - 71

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -

Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis

Abstract

Keywords

ASJC Scopus subject areas

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