TY - JOUR
T1 - Expression of AMPA-type glutamate receptors in HEK cells and cerebellar granule neurons impairs CXCL2-mediated chemotaxis
AU - Limatola, Cristina
AU - Di Bartolomeo, Sabrina
AU - Trettel, Flavia
AU - Lauro, Clotilde
AU - Ciotti, Maria T.
AU - Mercanti, Delio
AU - Castellani, Loriana
AU - Eusebi, Fabrizio
PY - 2003/1
Y1 - 2003/1
N2 - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.
AB - We find that cerebellar granule neurons (CGN) obtained from newborn rats (p3) migrate in response to both CXC chemokine ligand-2 (CXCL2) and -12 (CXCL12), while CGN from p7 rats are unresponsive to CXCL2. The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptor 1 (GluR1) greatly impairs the chemotaxis induced by CXCL2 in CXCR2-expressing HEK cells. By immunoprecipitation, we show that CXCR2 is associated with AMPA receptors (AMPARs) in p7 CGN, and with GluR1 co-expressed in HEK cells. Taken together, these results suggest that the association between CXCR2 and AMPARs results in the inhibition of CXCL2-dependent chemotaxis, and may represent a molecular mechanism underlying the modulation of nerve cell migration.
KW - Chemokines
KW - Chemotaxis
KW - Glutamate
KW - Rat cerebellar neurons
UR - http://www.scopus.com/inward/record.url?scp=0037219095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037219095&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(02)00401-0
DO - 10.1016/S0165-5728(02)00401-0
M3 - Article
C2 - 12507773
AN - SCOPUS:0037219095
VL - 134
SP - 61
EP - 71
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -