Expression of amyloid precursor protein isoforms in neuroectodermal cell lines upon retinoic acid exposure

F. Erba, E. Candi, M. Annicchiarico-Petruzzelli, P. Pagella, M. Brufani, G. Melino, F. Ascoli

Research output: Contribution to journalArticle

Abstract

Different neuroectodermal cell lines differentiated by retinoic acid express various protein isoforms of the amyloid precursor protein (APP). Human neuroblastoma [SK-N-BE(2), SK-N-SH, SH-SY5Y, CHP 100, SMS-KNR], and melanoma [SK-Mel 28, TVM-BO] cell lines produced two major APP of about 103 and 93 kDa. The glioblastoma line [LI] expresses only the larger APP isoform. With one exception, all cell lines showed only minor augmentation in intensity and ratio between the two isoforms after 5 days exposure to 5 μM retinoic acid. However, SH-SY5Y cells showed the induction of a smaller APP isoform of about 81 kDa accompanied by a relative reduction in intensity of the two larger proteins. These data were also confirmed by immunohistochemistry, which also showed no changes in intracellular localisation of APP, but with considerable cell-to-cell heterogeneity suggesting heterogeneous cell regulation.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalClinical Chemistry and Enzymology Communications
Volume6
Issue number1-2
Publication statusPublished - 1993

Keywords

  • βA4
  • Alzheimer
  • APP
  • Neuroblastoma

ASJC Scopus subject areas

  • Clinical Biochemistry

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