Expression of AP-2 transcription factor and of its downstream target genes c-kit, E-cadherin and p21 in human cutaneous melanoma

Alfonso Baldi, Daniele Santini, Tullio Battista, Emanuele Dragonetti, Giulio Ferranti, Tommasangelo Petitti, Angela M. Groeger, Anna Angelini, Raffaele Rossiello, Feliciano Baldi, Pier Giorgio Natali, Marco G. Paggi

Research output: Contribution to journalArticlepeer-review

Abstract

The AP-2 transcription factor plays a pivotal role in regulating the expression of several genes involved in tumor growth and progression of melanoma. We determined, by Western blot, variation in the level of expression of AP-2 and three of its downstream targets, c-kit, E-cadherin, and p21 in several human melanoma cell lines and, by immunohistochemistry, in a group of 99 histological samples including benign and malignat melanocytic lesions. A significant negative correlation between AP-2 expression level and tumor thickness was found. Moreover, AP-2 expression was positively associated with E-cadherin and c-kit expression. In contrast, there was a significant negative association between AP-2 and p21 expression levels. These findings suggest that p21 is independent of AP-2 transactivator function during the latest phases of melanoma progression. Finally, AP-2, c-kit, E-cadherin, and p21 expression levels did not show to be able to distinguish between dysplastic nevi and nevi without dysplasia. We conclude that changes in the expression of these proteins are involved in the later phases of melanoma progression, and may be responsible for the transition from local invasive melanoma to metastasis.

Original languageEnglish
Pages (from-to)364-372
Number of pages9
JournalJournal of Cellular Biochemistry
Volume83
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • AP-2
  • c-kit
  • E-cadherin
  • Human skin melanoma
  • Immunohistochemistry
  • p21

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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