Abstract
Basic Fibroblast Growth Factor (bFGF) is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. The basic Fibroblast Growth Factor Receptor (FGFR) family comprises at least 4 high affinity tyrosine kinase receptors that require syndecans for their function. Mounting evidence indicates that syndecans, that bind both bFGF and their FGFRs, will act as stimulators, whereas syndecans that only bind bFGF will act as inhibitors of signaling by sequestering the growth factor. Recent findings have highlighted the importance of syndecans in urological cancers. The aim of this study is to investigate the expression of bFGF, its receptors (R1 and R2) and syndecans (1-4) in invasive urothelial carcinoma and normal-looking urothelium by Western blotting, RT-PCR, and immunohistochemistry analyses. Interestingly, bFGF, FGFR1 and FGFR2 protein levels statistically increased in bladder cancer tissues. mRNA of FGFR1 and syndecans (1-4), showed a statistically significant increase while an mRNA increase in the other molecules analysed was not significant. bFGF, its receptors and syndecan immunostaining were mainly present in the urothelium both in normal-looking tissues and urothelial neoplastic cells. In conclusion, our data report that the bFGF, FGFR and syndecan expressions are altered in bladder tumours.
| Original language | English |
|---|---|
| Pages (from-to) | 627-638 |
| Number of pages | 12 |
| Journal | International Journal of Immunopathology and Pharmacology |
| Volume | 22 |
| Issue number | 3 |
| Publication status | Published - Jul 2009 |
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Keywords
- bFGF
- Bladder
- FGFRs
- Immunohistochemistry
- RT-PCR
- Syndecans
- Urothelial carcinoma
- Western blotting
ASJC Scopus subject areas
- Pharmacology
- Immunology
- Immunology and Allergy
Cite this
Expression of basic fibroblast growth factor, its receptors and syndecans in bladder cancer. / Marzioni, Daniela; Lorenzi, T.; Mazzucchelli, R.; Capparuccia, L.; Morroni, M.; Fiorini, R.; Bracalenti, C.; Catalano, A.; David, G.; Castellucci, M.; Muzzonigro, G.; Montironi, R.
In: International Journal of Immunopathology and Pharmacology, Vol. 22, No. 3, 07.2009, p. 627-638.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of basic fibroblast growth factor, its receptors and syndecans in bladder cancer
AU - Marzioni, Daniela
AU - Lorenzi, T.
AU - Mazzucchelli, R.
AU - Capparuccia, L.
AU - Morroni, M.
AU - Fiorini, R.
AU - Bracalenti, C.
AU - Catalano, A.
AU - David, G.
AU - Castellucci, M.
AU - Muzzonigro, G.
AU - Montironi, R.
PY - 2009/7
Y1 - 2009/7
N2 - Basic Fibroblast Growth Factor (bFGF) is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. The basic Fibroblast Growth Factor Receptor (FGFR) family comprises at least 4 high affinity tyrosine kinase receptors that require syndecans for their function. Mounting evidence indicates that syndecans, that bind both bFGF and their FGFRs, will act as stimulators, whereas syndecans that only bind bFGF will act as inhibitors of signaling by sequestering the growth factor. Recent findings have highlighted the importance of syndecans in urological cancers. The aim of this study is to investigate the expression of bFGF, its receptors (R1 and R2) and syndecans (1-4) in invasive urothelial carcinoma and normal-looking urothelium by Western blotting, RT-PCR, and immunohistochemistry analyses. Interestingly, bFGF, FGFR1 and FGFR2 protein levels statistically increased in bladder cancer tissues. mRNA of FGFR1 and syndecans (1-4), showed a statistically significant increase while an mRNA increase in the other molecules analysed was not significant. bFGF, its receptors and syndecan immunostaining were mainly present in the urothelium both in normal-looking tissues and urothelial neoplastic cells. In conclusion, our data report that the bFGF, FGFR and syndecan expressions are altered in bladder tumours.
AB - Basic Fibroblast Growth Factor (bFGF) is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. The basic Fibroblast Growth Factor Receptor (FGFR) family comprises at least 4 high affinity tyrosine kinase receptors that require syndecans for their function. Mounting evidence indicates that syndecans, that bind both bFGF and their FGFRs, will act as stimulators, whereas syndecans that only bind bFGF will act as inhibitors of signaling by sequestering the growth factor. Recent findings have highlighted the importance of syndecans in urological cancers. The aim of this study is to investigate the expression of bFGF, its receptors (R1 and R2) and syndecans (1-4) in invasive urothelial carcinoma and normal-looking urothelium by Western blotting, RT-PCR, and immunohistochemistry analyses. Interestingly, bFGF, FGFR1 and FGFR2 protein levels statistically increased in bladder cancer tissues. mRNA of FGFR1 and syndecans (1-4), showed a statistically significant increase while an mRNA increase in the other molecules analysed was not significant. bFGF, its receptors and syndecan immunostaining were mainly present in the urothelium both in normal-looking tissues and urothelial neoplastic cells. In conclusion, our data report that the bFGF, FGFR and syndecan expressions are altered in bladder tumours.
KW - bFGF
KW - Bladder
KW - FGFRs
KW - Immunohistochemistry
KW - RT-PCR
KW - Syndecans
KW - Urothelial carcinoma
KW - Western blotting
UR - http://www.scopus.com/inward/record.url?scp=70350551947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350551947&partnerID=8YFLogxK
M3 - Article
C2 - 19822079
AN - SCOPUS:70350551947
VL - 22
SP - 627
EP - 638
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
SN - 0394-6320
IS - 3
ER -