Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development

Luca Gentile, Vittorio Sebastiano, Valeria Merico, Manuela Monti, Maurizio Zuccotti, Silvia Garagna, Carlo Alberto Redi

Research output: Contribution to journalArticle

Abstract

The metabolism of fatty acids represents an important energy source for early embryonic development. Our knowledge of the molecular basis that regulate this metabolism is very poor. In this study we have analysed the pattern of expression of Cpt-1 and Cpt-2 genes, that encode the enzymes CPT-1 and CPT-2, which play a role in long chain fatty acids transport inside the mitochondria. The data obtained from the RT-PCR analysis show a rapid reduction in the number of transcripts from antral oocyte to ovulated MII oocyte and a significant difference between the expression profiles of Cpt-1 and Cpt-2 genes during preimplantation development. Cpt-1 is first temporarily expressed at the G2 phase of 2-cell embryo, it disappears at the 4-cell stage and reappears consistently between the 8-cell and morula stage; Cpt-2 is expressed from the MII oocyte, decreases to very low level at the 8-cell stage and is actively transcribed from morula, where it abruptly increases its expression to reach its maximum at the blastocyst stage. Interestingly, the high expression of both Cpt-1 and Cpt-2 at the morula stage is correlated with an increasing profile of oxygen uptake and fatty acid oxidation found at this stage of development. Furthermore, this rise of transcription is concomitant with other important epigenetic changes occurring prior to embryo cavitation and involving a reorganisation of chromatin structure and DNA methylation.

Original languageEnglish
Pages (from-to)217-229
Number of pages13
JournalRendiconti Lincei
Volume14
Issue number3
Publication statusPublished - 2003

Fingerprint

carnitine palmitoyltransferase
Carnitine
Transferases
Fatty acids
Morula
morula
fatty acid
Oocytes
Metabolism
oocytes
Fatty Acids
embryo
mice
Genes
metabolism
embryo (animal)
Mitochondria
Embryonic Structures
gene
cavitation

Keywords

  • Fatty acids metabolism
  • Gene expression
  • Preimplantation development
  • Retro Transcriptase-Polymerase chain reaction

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Gentile, L., Sebastiano, V., Merico, V., Monti, M., Zuccotti, M., Garagna, S., & Redi, C. A. (2003). Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development. Rendiconti Lincei, 14(3), 217-229.

Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development. / Gentile, Luca; Sebastiano, Vittorio; Merico, Valeria; Monti, Manuela; Zuccotti, Maurizio; Garagna, Silvia; Redi, Carlo Alberto.

In: Rendiconti Lincei, Vol. 14, No. 3, 2003, p. 217-229.

Research output: Contribution to journalArticle

Gentile, L, Sebastiano, V, Merico, V, Monti, M, Zuccotti, M, Garagna, S & Redi, CA 2003, 'Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development', Rendiconti Lincei, vol. 14, no. 3, pp. 217-229.
Gentile L, Sebastiano V, Merico V, Monti M, Zuccotti M, Garagna S et al. Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development. Rendiconti Lincei. 2003;14(3):217-229.
Gentile, Luca ; Sebastiano, Vittorio ; Merico, Valeria ; Monti, Manuela ; Zuccotti, Maurizio ; Garagna, Silvia ; Redi, Carlo Alberto. / Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development. In: Rendiconti Lincei. 2003 ; Vol. 14, No. 3. pp. 217-229.
@article{b0a7da15e0cf4c1ea8db9d2f7c151ad2,
title = "Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development",
abstract = "The metabolism of fatty acids represents an important energy source for early embryonic development. Our knowledge of the molecular basis that regulate this metabolism is very poor. In this study we have analysed the pattern of expression of Cpt-1 and Cpt-2 genes, that encode the enzymes CPT-1 and CPT-2, which play a role in long chain fatty acids transport inside the mitochondria. The data obtained from the RT-PCR analysis show a rapid reduction in the number of transcripts from antral oocyte to ovulated MII oocyte and a significant difference between the expression profiles of Cpt-1 and Cpt-2 genes during preimplantation development. Cpt-1 is first temporarily expressed at the G2 phase of 2-cell embryo, it disappears at the 4-cell stage and reappears consistently between the 8-cell and morula stage; Cpt-2 is expressed from the MII oocyte, decreases to very low level at the 8-cell stage and is actively transcribed from morula, where it abruptly increases its expression to reach its maximum at the blastocyst stage. Interestingly, the high expression of both Cpt-1 and Cpt-2 at the morula stage is correlated with an increasing profile of oxygen uptake and fatty acid oxidation found at this stage of development. Furthermore, this rise of transcription is concomitant with other important epigenetic changes occurring prior to embryo cavitation and involving a reorganisation of chromatin structure and DNA methylation.",
keywords = "Fatty acids metabolism, Gene expression, Preimplantation development, Retro Transcriptase-Polymerase chain reaction",
author = "Luca Gentile and Vittorio Sebastiano and Valeria Merico and Manuela Monti and Maurizio Zuccotti and Silvia Garagna and Redi, {Carlo Alberto}",
year = "2003",
language = "English",
volume = "14",
pages = "217--229",
journal = "ATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali",
issn = "2037-4631",
publisher = "Accademia Nazionale dei Lincei",
number = "3",

}

TY - JOUR

T1 - Expression of carnitine palmitoyl-transferase 1 and 2 during mouse preimplantation development

AU - Gentile, Luca

AU - Sebastiano, Vittorio

AU - Merico, Valeria

AU - Monti, Manuela

AU - Zuccotti, Maurizio

AU - Garagna, Silvia

AU - Redi, Carlo Alberto

PY - 2003

Y1 - 2003

N2 - The metabolism of fatty acids represents an important energy source for early embryonic development. Our knowledge of the molecular basis that regulate this metabolism is very poor. In this study we have analysed the pattern of expression of Cpt-1 and Cpt-2 genes, that encode the enzymes CPT-1 and CPT-2, which play a role in long chain fatty acids transport inside the mitochondria. The data obtained from the RT-PCR analysis show a rapid reduction in the number of transcripts from antral oocyte to ovulated MII oocyte and a significant difference between the expression profiles of Cpt-1 and Cpt-2 genes during preimplantation development. Cpt-1 is first temporarily expressed at the G2 phase of 2-cell embryo, it disappears at the 4-cell stage and reappears consistently between the 8-cell and morula stage; Cpt-2 is expressed from the MII oocyte, decreases to very low level at the 8-cell stage and is actively transcribed from morula, where it abruptly increases its expression to reach its maximum at the blastocyst stage. Interestingly, the high expression of both Cpt-1 and Cpt-2 at the morula stage is correlated with an increasing profile of oxygen uptake and fatty acid oxidation found at this stage of development. Furthermore, this rise of transcription is concomitant with other important epigenetic changes occurring prior to embryo cavitation and involving a reorganisation of chromatin structure and DNA methylation.

AB - The metabolism of fatty acids represents an important energy source for early embryonic development. Our knowledge of the molecular basis that regulate this metabolism is very poor. In this study we have analysed the pattern of expression of Cpt-1 and Cpt-2 genes, that encode the enzymes CPT-1 and CPT-2, which play a role in long chain fatty acids transport inside the mitochondria. The data obtained from the RT-PCR analysis show a rapid reduction in the number of transcripts from antral oocyte to ovulated MII oocyte and a significant difference between the expression profiles of Cpt-1 and Cpt-2 genes during preimplantation development. Cpt-1 is first temporarily expressed at the G2 phase of 2-cell embryo, it disappears at the 4-cell stage and reappears consistently between the 8-cell and morula stage; Cpt-2 is expressed from the MII oocyte, decreases to very low level at the 8-cell stage and is actively transcribed from morula, where it abruptly increases its expression to reach its maximum at the blastocyst stage. Interestingly, the high expression of both Cpt-1 and Cpt-2 at the morula stage is correlated with an increasing profile of oxygen uptake and fatty acid oxidation found at this stage of development. Furthermore, this rise of transcription is concomitant with other important epigenetic changes occurring prior to embryo cavitation and involving a reorganisation of chromatin structure and DNA methylation.

KW - Fatty acids metabolism

KW - Gene expression

KW - Preimplantation development

KW - Retro Transcriptase-Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=9744223590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9744223590&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 217

EP - 229

JO - ATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali

JF - ATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali

SN - 2037-4631

IS - 3

ER -