Abstract
Our aim was to evaluate in oral squamous cell carcinoma (OSCC) the relationship between some cell cycle markers and HPV infection, conditionally to age, gender and certain habits of patients, and to assess the ability of fuzzy neural networks (FNNs) in building up an adequate predictive model based on logic inference rules. Eighteen cases of OSCC were examined by immunohistochemistry for MIB-1, PCNA and survivin expression; presence of HPV DNA was investigated in exfoliated oral mucosa cells by nested PCR (nPCR, MY09-MY11/GP5-GP6), and HPV genotype was determined by direct DNA sequencing. Data were analyzed by traditional statistics (TS) and FNNs. HPV DNA was found in 9/18 OSCCs (50.0%) without any significant higher risk of HPV infection with respect to the sociodemographic variables considered (p > 0.2), apart from tobacco smoking, reported in 44.4% of OSCC HPV-positive vs. 100% HPV-negative subjects (p = 0.029). Regarding cell cycle markers, TS and FNN revealed that survivin was expressed significantly more in HPV-negative than in HPV-positive OSCC [root mean-square error (RMSE) = 5.89 × 10-6, % predicted 100.0]; furthermore, smoking played a protective role for survivin expression in HPV-positive cases (OR = 0.019, 95% CI 0.001-0.723, RMSE = 0.20, % of prevision 94.4). FNN, although on a small sample size, allowed us to confirm data by TS and to hypothesize a different cell cycle pattern for HPV-positive vs. HPV-negative OSCC. In the latter cases, the relevance of apoptotic vs. proliferative markers suggested that they may be related to the different supposed outcome of HPV-negative OSCC and that HPV may have a protective role in the expression level of survivin, especially in tobacco smokers.
| Original language | English |
|---|---|
| Pages (from-to) | 717-723 |
| Number of pages | 7 |
| Journal | International Journal of Cancer |
| Volume | 115 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jul 10 2005 |
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Keywords
- Carcinogenesis
- Fuzzy neural network
- Human papillomavirus
- Oral squamous cell carcinoma
- Survivin
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Expression of cell cycle markers and human papillomavirus infection in oral squamous cell carcinoma : Use of fuzzy neural networks. / Lo Muzio, Lorenzo; D'Angelo, Matteo; Procaccini, Maurizio; Bambini, Fabrizio; Calvino, Francesco; Florena, Ada Maria; Franco, Vito; Giovannelli, Lucia; Ammatuna, Pietro; Campisi, Giuseppina.
In: International Journal of Cancer, Vol. 115, No. 5, 10.07.2005, p. 717-723.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of cell cycle markers and human papillomavirus infection in oral squamous cell carcinoma
T2 - Use of fuzzy neural networks
AU - Lo Muzio, Lorenzo
AU - D'Angelo, Matteo
AU - Procaccini, Maurizio
AU - Bambini, Fabrizio
AU - Calvino, Francesco
AU - Florena, Ada Maria
AU - Franco, Vito
AU - Giovannelli, Lucia
AU - Ammatuna, Pietro
AU - Campisi, Giuseppina
PY - 2005/7/10
Y1 - 2005/7/10
N2 - Our aim was to evaluate in oral squamous cell carcinoma (OSCC) the relationship between some cell cycle markers and HPV infection, conditionally to age, gender and certain habits of patients, and to assess the ability of fuzzy neural networks (FNNs) in building up an adequate predictive model based on logic inference rules. Eighteen cases of OSCC were examined by immunohistochemistry for MIB-1, PCNA and survivin expression; presence of HPV DNA was investigated in exfoliated oral mucosa cells by nested PCR (nPCR, MY09-MY11/GP5-GP6), and HPV genotype was determined by direct DNA sequencing. Data were analyzed by traditional statistics (TS) and FNNs. HPV DNA was found in 9/18 OSCCs (50.0%) without any significant higher risk of HPV infection with respect to the sociodemographic variables considered (p > 0.2), apart from tobacco smoking, reported in 44.4% of OSCC HPV-positive vs. 100% HPV-negative subjects (p = 0.029). Regarding cell cycle markers, TS and FNN revealed that survivin was expressed significantly more in HPV-negative than in HPV-positive OSCC [root mean-square error (RMSE) = 5.89 × 10-6, % predicted 100.0]; furthermore, smoking played a protective role for survivin expression in HPV-positive cases (OR = 0.019, 95% CI 0.001-0.723, RMSE = 0.20, % of prevision 94.4). FNN, although on a small sample size, allowed us to confirm data by TS and to hypothesize a different cell cycle pattern for HPV-positive vs. HPV-negative OSCC. In the latter cases, the relevance of apoptotic vs. proliferative markers suggested that they may be related to the different supposed outcome of HPV-negative OSCC and that HPV may have a protective role in the expression level of survivin, especially in tobacco smokers.
AB - Our aim was to evaluate in oral squamous cell carcinoma (OSCC) the relationship between some cell cycle markers and HPV infection, conditionally to age, gender and certain habits of patients, and to assess the ability of fuzzy neural networks (FNNs) in building up an adequate predictive model based on logic inference rules. Eighteen cases of OSCC were examined by immunohistochemistry for MIB-1, PCNA and survivin expression; presence of HPV DNA was investigated in exfoliated oral mucosa cells by nested PCR (nPCR, MY09-MY11/GP5-GP6), and HPV genotype was determined by direct DNA sequencing. Data were analyzed by traditional statistics (TS) and FNNs. HPV DNA was found in 9/18 OSCCs (50.0%) without any significant higher risk of HPV infection with respect to the sociodemographic variables considered (p > 0.2), apart from tobacco smoking, reported in 44.4% of OSCC HPV-positive vs. 100% HPV-negative subjects (p = 0.029). Regarding cell cycle markers, TS and FNN revealed that survivin was expressed significantly more in HPV-negative than in HPV-positive OSCC [root mean-square error (RMSE) = 5.89 × 10-6, % predicted 100.0]; furthermore, smoking played a protective role for survivin expression in HPV-positive cases (OR = 0.019, 95% CI 0.001-0.723, RMSE = 0.20, % of prevision 94.4). FNN, although on a small sample size, allowed us to confirm data by TS and to hypothesize a different cell cycle pattern for HPV-positive vs. HPV-negative OSCC. In the latter cases, the relevance of apoptotic vs. proliferative markers suggested that they may be related to the different supposed outcome of HPV-negative OSCC and that HPV may have a protective role in the expression level of survivin, especially in tobacco smokers.
KW - Carcinogenesis
KW - Fuzzy neural network
KW - Human papillomavirus
KW - Oral squamous cell carcinoma
KW - Survivin
UR - http://www.scopus.com/inward/record.url?scp=20144379263&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20144379263&partnerID=8YFLogxK
U2 - 10.1002/ijc.20940
DO - 10.1002/ijc.20940
M3 - Article
C2 - 15729691
AN - SCOPUS:20144379263
VL - 115
SP - 717
EP - 723
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 5
ER -