Expression of cell cycle regulatory proteins and analysis of apoptosis in normal nasal mucosa and in nasal polyps

Werner Garavello, Paola Viganò, Marco Romagnoli, Lorenza Sordo, Emilio Berti, Giovanni Tredici, Renato Maria Gaini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: The etiopathogenesis of nasal polyps still is to be clarified. Although hyperplasia is a typical feature of these pathological processes, little attention has been paid to specific aspects of cellular growth in polyps. We have evaluated the expression and localization of some of the regulatory proteins that direct the cell through the specific sequence of events culminating in mitosis or apoptosis in nasal polyps. Methods: Twenty samples of nasal polyps and 20 samples of normal nasal mucosa have been analyzed for apoptotic index by detecting the DNA 3′ OH ends deriving from DNA fragmentation. Moreover, they have been evaluated by immunohistochemical staining for expression of Ki-67, cyclins A and B1, p53, p21, p27, murine double minute clone 2, and Bcl-2. Results: We have identified a greater proportion of proliferating cells in the lining epithelial cells of the polyps when compared with the normal mucosa as stained with anti-Ki-67 antibodies. An overexpression of p53, MDM2, and Bcl-2 and an increased apoptosis were observed in nasal polyps compared with the normal mucosa, whereas no variation of p27 expression was observed. The p21 and eyclins A and B1 were rarely expressed in both pathological and normal tissue. Conclusion: The p53-based control system of cell cycle progression appears to be altered in nasal polyps, potentially leading to an abrogation of the DNA damage checkpoint. Evaluation of the expression of the regulatory proteins that direct the cells throughout their cycle in nasal polyps may allow a better understanding of the biological behavior and clinical outcome of these benign pathological entities.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalAmerican Journal of Rhinology
Volume19
Issue number6
Publication statusPublished - Nov 2005

Fingerprint

Nasal Polyps
Cell Cycle Proteins
Nasal Mucosa
Apoptosis
Polyps
Mucous Membrane
Cyclin B1
Cyclin A
DNA Fragmentation
Pathologic Processes
Cell Cycle Checkpoints
Mitosis
DNA Damage
Hyperplasia
Proteins
Clone Cells
Epithelial Cells
Staining and Labeling
Antibodies
DNA

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression of cell cycle regulatory proteins and analysis of apoptosis in normal nasal mucosa and in nasal polyps. / Garavello, Werner; Viganò, Paola; Romagnoli, Marco; Sordo, Lorenza; Berti, Emilio; Tredici, Giovanni; Gaini, Renato Maria.

In: American Journal of Rhinology, Vol. 19, No. 6, 11.2005, p. 549-553.

Research output: Contribution to journalArticle

Garavello, Werner ; Viganò, Paola ; Romagnoli, Marco ; Sordo, Lorenza ; Berti, Emilio ; Tredici, Giovanni ; Gaini, Renato Maria. / Expression of cell cycle regulatory proteins and analysis of apoptosis in normal nasal mucosa and in nasal polyps. In: American Journal of Rhinology. 2005 ; Vol. 19, No. 6. pp. 549-553.
@article{3ea7754beac74b009bee7294d3ecb709,
title = "Expression of cell cycle regulatory proteins and analysis of apoptosis in normal nasal mucosa and in nasal polyps",
abstract = "Background: The etiopathogenesis of nasal polyps still is to be clarified. Although hyperplasia is a typical feature of these pathological processes, little attention has been paid to specific aspects of cellular growth in polyps. We have evaluated the expression and localization of some of the regulatory proteins that direct the cell through the specific sequence of events culminating in mitosis or apoptosis in nasal polyps. Methods: Twenty samples of nasal polyps and 20 samples of normal nasal mucosa have been analyzed for apoptotic index by detecting the DNA 3′ OH ends deriving from DNA fragmentation. Moreover, they have been evaluated by immunohistochemical staining for expression of Ki-67, cyclins A and B1, p53, p21, p27, murine double minute clone 2, and Bcl-2. Results: We have identified a greater proportion of proliferating cells in the lining epithelial cells of the polyps when compared with the normal mucosa as stained with anti-Ki-67 antibodies. An overexpression of p53, MDM2, and Bcl-2 and an increased apoptosis were observed in nasal polyps compared with the normal mucosa, whereas no variation of p27 expression was observed. The p21 and eyclins A and B1 were rarely expressed in both pathological and normal tissue. Conclusion: The p53-based control system of cell cycle progression appears to be altered in nasal polyps, potentially leading to an abrogation of the DNA damage checkpoint. Evaluation of the expression of the regulatory proteins that direct the cells throughout their cycle in nasal polyps may allow a better understanding of the biological behavior and clinical outcome of these benign pathological entities.",
author = "Werner Garavello and Paola Vigan{\`o} and Marco Romagnoli and Lorenza Sordo and Emilio Berti and Giovanni Tredici and Gaini, {Renato Maria}",
year = "2005",
month = "11",
language = "English",
volume = "19",
pages = "549--553",
journal = "American Journal of Rhinology",
issn = "1050-6586",
publisher = "OceanSide Publications Inc.",
number = "6",

}

TY - JOUR

T1 - Expression of cell cycle regulatory proteins and analysis of apoptosis in normal nasal mucosa and in nasal polyps

AU - Garavello, Werner

AU - Viganò, Paola

AU - Romagnoli, Marco

AU - Sordo, Lorenza

AU - Berti, Emilio

AU - Tredici, Giovanni

AU - Gaini, Renato Maria

PY - 2005/11

Y1 - 2005/11

N2 - Background: The etiopathogenesis of nasal polyps still is to be clarified. Although hyperplasia is a typical feature of these pathological processes, little attention has been paid to specific aspects of cellular growth in polyps. We have evaluated the expression and localization of some of the regulatory proteins that direct the cell through the specific sequence of events culminating in mitosis or apoptosis in nasal polyps. Methods: Twenty samples of nasal polyps and 20 samples of normal nasal mucosa have been analyzed for apoptotic index by detecting the DNA 3′ OH ends deriving from DNA fragmentation. Moreover, they have been evaluated by immunohistochemical staining for expression of Ki-67, cyclins A and B1, p53, p21, p27, murine double minute clone 2, and Bcl-2. Results: We have identified a greater proportion of proliferating cells in the lining epithelial cells of the polyps when compared with the normal mucosa as stained with anti-Ki-67 antibodies. An overexpression of p53, MDM2, and Bcl-2 and an increased apoptosis were observed in nasal polyps compared with the normal mucosa, whereas no variation of p27 expression was observed. The p21 and eyclins A and B1 were rarely expressed in both pathological and normal tissue. Conclusion: The p53-based control system of cell cycle progression appears to be altered in nasal polyps, potentially leading to an abrogation of the DNA damage checkpoint. Evaluation of the expression of the regulatory proteins that direct the cells throughout their cycle in nasal polyps may allow a better understanding of the biological behavior and clinical outcome of these benign pathological entities.

AB - Background: The etiopathogenesis of nasal polyps still is to be clarified. Although hyperplasia is a typical feature of these pathological processes, little attention has been paid to specific aspects of cellular growth in polyps. We have evaluated the expression and localization of some of the regulatory proteins that direct the cell through the specific sequence of events culminating in mitosis or apoptosis in nasal polyps. Methods: Twenty samples of nasal polyps and 20 samples of normal nasal mucosa have been analyzed for apoptotic index by detecting the DNA 3′ OH ends deriving from DNA fragmentation. Moreover, they have been evaluated by immunohistochemical staining for expression of Ki-67, cyclins A and B1, p53, p21, p27, murine double minute clone 2, and Bcl-2. Results: We have identified a greater proportion of proliferating cells in the lining epithelial cells of the polyps when compared with the normal mucosa as stained with anti-Ki-67 antibodies. An overexpression of p53, MDM2, and Bcl-2 and an increased apoptosis were observed in nasal polyps compared with the normal mucosa, whereas no variation of p27 expression was observed. The p21 and eyclins A and B1 were rarely expressed in both pathological and normal tissue. Conclusion: The p53-based control system of cell cycle progression appears to be altered in nasal polyps, potentially leading to an abrogation of the DNA damage checkpoint. Evaluation of the expression of the regulatory proteins that direct the cells throughout their cycle in nasal polyps may allow a better understanding of the biological behavior and clinical outcome of these benign pathological entities.

UR - http://www.scopus.com/inward/record.url?scp=30944457775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30944457775&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 549

EP - 553

JO - American Journal of Rhinology

JF - American Journal of Rhinology

SN - 1050-6586

IS - 6

ER -