Expression of chromosomal proteins HMG-14 and HMG-17 in transformed human cells

Massimo P. Crippa, James M. Pash, Brenda I. Gerwin, Thomas E. Smithgall, Robert I. Glazer, Michael Bustin

Research output: Contribution to journalArticle

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Abstract

The relation between cellular phenotype and expression of chromosomal high mobility group proteins 14 and 17 (HMG-14 and HMG-17) has been examined in human cell lineages. Quantitation of HMG-14 and HMG-17 mRNA in several human cell lines revealed differences in both the steady state mRNA level and in the ratio of HMG-14 to HMG-17 mRNA. Analysis of phenotypically distinct derivatives of human bronchial epithelial cells revealed small differences between both the steady state mRNA levels and the relative amount of these proteins among the clonal variants. The effect of myeloid differentiation on the mRNA level of HMG-14 and HMG-17 was examined in the human promyelocytic leukemia cell line HL-60 following treatment with several granulocytic and monocytic differentiating agents. The ratio of HMG-17 mRNA to either HMG-14 or histone H4 mRNA varied among the cell phenotypes suggesting that phenotype switching may result in detectable alterations in the expression of the HMG-14 and HMG-17 genes. The data suggest that, although the ratio of HMG-14 to HMG-17 mRNA varies among human cell lines, these variations are relatively small.

Original languageEnglish
Pages (from-to)2022-2026
Number of pages5
JournalCancer Research
Volume50
Issue number7
Publication statusPublished - Apr 1 1990

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HMGN2 Protein
HMGN1 Protein
Messenger RNA
Phenotype
Cell Line
High Mobility Group Proteins
Cell Lineage
Histones
Leukemia
Epithelial Cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Crippa, M. P., Pash, J. M., Gerwin, B. I., Smithgall, T. E., Glazer, R. I., & Bustin, M. (1990). Expression of chromosomal proteins HMG-14 and HMG-17 in transformed human cells. Cancer Research, 50(7), 2022-2026.

Expression of chromosomal proteins HMG-14 and HMG-17 in transformed human cells. / Crippa, Massimo P.; Pash, James M.; Gerwin, Brenda I.; Smithgall, Thomas E.; Glazer, Robert I.; Bustin, Michael.

In: Cancer Research, Vol. 50, No. 7, 01.04.1990, p. 2022-2026.

Research output: Contribution to journalArticle

Crippa, MP, Pash, JM, Gerwin, BI, Smithgall, TE, Glazer, RI & Bustin, M 1990, 'Expression of chromosomal proteins HMG-14 and HMG-17 in transformed human cells', Cancer Research, vol. 50, no. 7, pp. 2022-2026.
Crippa MP, Pash JM, Gerwin BI, Smithgall TE, Glazer RI, Bustin M. Expression of chromosomal proteins HMG-14 and HMG-17 in transformed human cells. Cancer Research. 1990 Apr 1;50(7):2022-2026.
Crippa, Massimo P. ; Pash, James M. ; Gerwin, Brenda I. ; Smithgall, Thomas E. ; Glazer, Robert I. ; Bustin, Michael. / Expression of chromosomal proteins HMG-14 and HMG-17 in transformed human cells. In: Cancer Research. 1990 ; Vol. 50, No. 7. pp. 2022-2026.
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