Expression of costimulatory molecules in human neuroblastoma. Evidence that CD40 + neuroblastoma cells undergo apoptosis following interaction with CD40L

I. Airoldi, S. Lualdi, S. Bruno, L. Raffaghello, M. Occhino, C. Gambini, V. Pistoia, M. V. Corrias

Research output: Contribution to journalArticlepeer-review

Abstract

Tumour cells display low to absent expression of costimulatory molecules. Here, we have investigated the expression of costimulatory molecules (CD40, CD80, CD86, PD-1L, B7H2, OX40L and 4-1BBL) in human neuroblastoma (NB) cells, since virtually no information is available on this issue. Both established NB cell lines and primary tumours were tested by RT-PCR and flow cytometry. Neuroblastoma cell lines expressed the transcripts of all costimulatory molecule genes, but not the corresponding proteins. Culture of NB cell lines with human recombinant (r)IFN-γ induced surface expression of CD40 in half of them. Primary NB cells showed CD40, CD80, CD86, OX40L, 4-1BBL, but not PD-1L and B7H2, mRNA expression. Surface CD40 was consistently detected on primary NB cells by flow cytometry. Interferon-γ gene-transfected NB cells expressed constitutively surface CD40 and were induced into apoptosis by incubation with rCD40L through a caspase-8-dependent mechanism. CD40 may represent a novel therapeutic target in NB.

Original languageEnglish
Pages (from-to)1527-1536
Number of pages10
JournalBritish Journal of Cancer
Volume88
Issue number10
DOIs
Publication statusPublished - May 19 2003

Keywords

  • Apoptosis
  • Caspase-8
  • CD40
  • Costimulatory molecules
  • Human neuroblastoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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