Expression of CXC chemokine receptors 1-5 and their ligands in human glioma tissues

Role of CXCR4 and SDF1 in glioma cell proliferation and migration

Adriana Bajetto, Federica Barbieri, Alessandra Dorcaratto, Simone Barbero, Antonio Daga, Carola Porcile, Jean Louis Ravetti, Gianluigi Zona, Renato Spaziante, Giorgio Corte, Gennaro Schettini, Tullio Florio

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Chemokines have been involved in cellular processes associated to malignant transformation such as proliferation, migration and angiogenesis. The expression of five CXC chemokine receptors and their main ligands was analysed by RT-PCR in 31 human astrocytic neoplasms. The mRNAs for all the receptors analysed were identified in a high percentage of tumours, while their ligands showed lower expression. CXCR4 and SDF1 were the most frequently mRNA identified (29/31 and 13/31 of the gliomas studied, respectively). Thus, we further analysed the cell localization of CXCR4 and SDF1 in immunohistochemistry experiments. We show a marked co-localization of CXCR4 and SDF1 in tumour cells, mainly evident in psudolpalisade and microcystic degeneration areas and in the vascular endothelium. In addition, hSDF1α induced a significant increase of DNA synthesis in primary human glioblastoma cell cultures and chemotaxis in a glioblastoma cell line. These results provide evidence of the expression of multiple CXC chemokines and their receptors in brain tumours and that in particular CXCR4 and SDF1 sustain proliferation and migration of glioma cells to promote malignant progression.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalNeurochemistry International
Volume49
Issue number5
DOIs
Publication statusPublished - Oct 2006

Fingerprint

CXCR5 Receptors
Interleukin-8A Receptors
CXCR Receptors
Glioma
Cell Movement
Cell Proliferation
Glioblastoma
Ligands
Neoplasms
Messenger RNA
Vascular Endothelium
Chemotaxis
Chemokines
Brain Neoplasms
Cell Culture Techniques
Immunohistochemistry
Cell Line
Polymerase Chain Reaction
DNA

Keywords

  • Cell migration
  • Cell proliferation
  • Chemokine
  • CXCR4
  • Glioma
  • SDF1

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Expression of CXC chemokine receptors 1-5 and their ligands in human glioma tissues : Role of CXCR4 and SDF1 in glioma cell proliferation and migration. / Bajetto, Adriana; Barbieri, Federica; Dorcaratto, Alessandra; Barbero, Simone; Daga, Antonio; Porcile, Carola; Ravetti, Jean Louis; Zona, Gianluigi; Spaziante, Renato; Corte, Giorgio; Schettini, Gennaro; Florio, Tullio.

In: Neurochemistry International, Vol. 49, No. 5, 10.2006, p. 423-432.

Research output: Contribution to journalArticle

Bajetto, A, Barbieri, F, Dorcaratto, A, Barbero, S, Daga, A, Porcile, C, Ravetti, JL, Zona, G, Spaziante, R, Corte, G, Schettini, G & Florio, T 2006, 'Expression of CXC chemokine receptors 1-5 and their ligands in human glioma tissues: Role of CXCR4 and SDF1 in glioma cell proliferation and migration', Neurochemistry International, vol. 49, no. 5, pp. 423-432. https://doi.org/10.1016/j.neuint.2006.03.003
Bajetto, Adriana ; Barbieri, Federica ; Dorcaratto, Alessandra ; Barbero, Simone ; Daga, Antonio ; Porcile, Carola ; Ravetti, Jean Louis ; Zona, Gianluigi ; Spaziante, Renato ; Corte, Giorgio ; Schettini, Gennaro ; Florio, Tullio. / Expression of CXC chemokine receptors 1-5 and their ligands in human glioma tissues : Role of CXCR4 and SDF1 in glioma cell proliferation and migration. In: Neurochemistry International. 2006 ; Vol. 49, No. 5. pp. 423-432.
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