Expression of cyclic nucleotide-gated channels in the rat medial vestibular nucleus

Maria V. Podda, Maria E. Marcocci, Barbara Del Carlo, Anna T. Palamara, Gian Battista Azzena, Claudio Grassi

Research output: Contribution to journalArticle

Abstract

The role of cyclic nucleotide-gated (CNG) channels in sensory signal transduction in retinal and olfactory cells is widely recognized, but there is increasing evidence that they also play more general functions in the central nervous system as downstream effectors of cyclic nucleotides. Here, we demonstrate the expression of the α-subunit of rod- and olfactory-type CNG channels (CNGI and CNG2, respectively) in the rat medial vestibular nucleus (MVN). Nested polymerase chain reaction revealed CNG channel mRNA in the MVN, and CNG1 and CNG2 proteins were also detected by Western blotting and immunohistochemistry. Finally, electrophysiological evidence is provided suggesting that CNG channels play a functional role in the MVN.

Original languageEnglish
Pages (from-to)1939-1943
Number of pages5
JournalNeuroReport
Volume16
Issue number17
DOIs
Publication statusPublished - Nov 28 2005

Keywords

  • Brainstem slices
  • cAMP
  • cGMP
  • CNG-immunoreactivity
  • Cyclic nucleotide-gated channels
  • Extracellular recordings
  • Medial vestibular nucleus
  • Nested PCR

ASJC Scopus subject areas

  • Neuroscience(all)

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