Expression of different chemokines by human osteosarcoma cells in response to tumor necrosis factor-α

Brunella Grigolo, Livia Roseti, Gina Lisignoli, Giulia Remiddi, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

We have investigated the expression of different chemokines in the surnatants and inside the cells of four human osteosarcoma cell lines. HOS, U-2 OS, MG63 and Saos-2 cells were cultured for 24, 48, 72 hours both in basal conditions and after stimulus with TNFα. Human stromal cells were used as control. IL-8 and MCP-1 are present in higher concentration in the surnatants in contrast to RANTES which is present primarily inside the cells. IL-8 and MCP-1 are not totally expressed by all the human osteosarcoma cell lines in unstimulated conditions, but became detectable after TNFα treatment. In general, this cytokine stimulated the production and release of the three chemokines.

Original languageEnglish
Pages (from-to)3093-3098
Number of pages6
JournalAnticancer Research
Volume19
Issue number4 B
Publication statusPublished - 1999

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Keywords

  • Chemokines
  • Osteosarcoma cells
  • TNFα

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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