Expression of Egr-1 correlates with the transformed phenotype and the type of viral latency in EBV genome positive lymphoid cell lines

A. Calogero, L. Cuomo, M. D'Onofrio, U. De Grazia, P. Spinsanti, D. Mercola, A. Faggioni, L. Frati, E. D. Adamson, G. Ragona

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Abstract

In this paper we have investigated the role of Egr-1 in B cell growth regulation by examining the gene expression in a panel of B cell lines, including both EBV genome negative and EBV carrying cell lines. Egr-1 expression correlates with the cellular phenotype and the specific pattern of viral latency established within the individual cell lines. Thus, constitutive activation of Egr-1 gene is invariably associated with unrestricted expression of viral latent genes in all group m EBV genome carrying cell lines. In contrast, Egr-1 expression is abrogated in group I Burkitt tumor cells, irrespective of the EBV genome carrying status. Activated viral gene expression associated with phenotypic conversion of group I cell lines in to group II or III restores the Egr-1 gene expression. Several forms of EGR-1 protein are found within the different groups of cell lines, and the binding activity to DNA consensus sequences was investigated. Finally, time course analysis of Egr-1 expression during the early steps of EBV infection in vitro demonstrated that Egr-1 is upregulated within minutes from the initial interaction with the B lymphocyte.

Original languageEnglish
Pages (from-to)2105-2112
Number of pages8
JournalOncogene
Volume13
Issue number10
Publication statusPublished - 1996

Keywords

  • EBV
  • Egr-1
  • Lymphoid cell lines

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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    Calogero, A., Cuomo, L., D'Onofrio, M., De Grazia, U., Spinsanti, P., Mercola, D., Faggioni, A., Frati, L., Adamson, E. D., & Ragona, G. (1996). Expression of Egr-1 correlates with the transformed phenotype and the type of viral latency in EBV genome positive lymphoid cell lines. Oncogene, 13(10), 2105-2112.