Expression of fatty acid amide hydrolase (FAAH) in human, mouse, and rat urinary bladder and effects of faah inhibition on bladder function in awake rats

Frank Strittmatter, Giorgio Gandaglia, Fabio Benigni, Arianna Bettiga, Patrizio Rigatti, Francesco Montorsi, Christian Gratzke, Christian Stief, Giorgia Colciago, Petter Hedlund

Research output: Contribution to journalArticle

Abstract

Background: Cannabinoid receptor (CB)-mediated functions may be involved in the regulation of bladder function, but information on endocannabinoid signals during micturition is scarce. Objective: Investigate the expression of the endocannabinoid-degrading enzyme fatty acid amide hydrolase (FAAH) in human, rat, and mouse bladders and study the effects of inhibition of FAAH during urodynamics in awake rats. Design, setting, and participants: Bladder tissue from humans, mice, and rats was used for measurements. Female Sprague-Dawley rats were administered the FAAH inhibitor oleoyl ethyl amide (OEtA) or vehicle intravenously (IV) or intravesically (IVES) with or without rimonabant (CB1 antagonist) or SR144528 (CB2 antagonist). Measurements: Real-time transcriptase-polymerase chain reaction, Western blot, immunohistochemistry, and cystometry in awake rats. Results and limitations: Messenger RNA and protein for FAAH was expressed in the mucosa of human, mouse, and rat urinary bladders. Immunoreactivities for FAAH and CB2 were codistributed in rat and human urothelium. IV OEtA (0.3 mg/kg) to rats increased intercontraction intervals (ICIs), micturition volume (MV), bladder capacity (BC), and threshold pressure (TP) by 17 ± 1%, 16 ± 1%, 17 ± 1%, and 19 ± 5%, respectively (all p <0.05 vs baseline). IVES OEtA (1 and 10 mg/l) in rats dose-dependently increased (p <0.05 vs baseline) ICI (19 ± 2% and 35 ± 5%), MV (15 ± 3% and 32 ± 4%), BC (16 ± 2% and 34 ± 4%), and TP (15 ± 1%, 21 ± 3%). SR144528 (IVES 5 mg/l) abolished all effects of OEtA, whereas rimonabant only counteracted effects of OEtA on TP. Conclusions: Bladder mucosa of all species expressed FAAH. Rat and human urothelium coexpressed FAAH and CB2. The FAAH inhibitor OEtA altered urodynamic parameters that reflect sensory functions of micturition in rats. Suggesting a role for the endocannabinoid system in bladder mechanoafferent functions of rats, effects of IVES OEtA were abolished by an IVES CB2 antagonist and partly counteracted by an IVES CB1 antagonist.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalEuropean Urology
Volume61
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords

  • CB1
  • CB2
  • Cystometry
  • Endocannabinoid
  • Enzyme
  • Intravesical
  • mRNA
  • Mucosa
  • Protein

ASJC Scopus subject areas

  • Urology

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