Abstract
The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from α-galactosylceramide (α-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins.In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.
Original language | English |
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Pages (from-to) | 420-425 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 398 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jul 2010 |
Keywords
- Cytokines
- GPR35
- Human iNKT cells
- Kynurenic acid
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology