Abstract
The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from α-galactosylceramide (α-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins.In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.
| Original language | English |
|---|---|
| Pages (from-to) | 420-425 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 398 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 2010 |
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Keywords
- Cytokines
- GPR35
- Human iNKT cells
- Kynurenic acid
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
Expression of functional GPR35 in human iNKT cells. / Fallarini, Silvia; Magliulo, Laura; Paoletti, Tiziana; de Lalla, Claudia; Lombardi, Grazia.
In: Biochemical and Biophysical Research Communications, Vol. 398, No. 3, 07.2010, p. 420-425.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of functional GPR35 in human iNKT cells
AU - Fallarini, Silvia
AU - Magliulo, Laura
AU - Paoletti, Tiziana
AU - de Lalla, Claudia
AU - Lombardi, Grazia
PY - 2010/7
Y1 - 2010/7
N2 - The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from α-galactosylceramide (α-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins.In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.
AB - The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from α-galactosylceramide (α-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins.In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.
KW - Cytokines
KW - GPR35
KW - Human iNKT cells
KW - Kynurenic acid
UR - http://www.scopus.com/inward/record.url?scp=77955278967&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955278967&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2010.06.091
DO - 10.1016/j.bbrc.2010.06.091
M3 - Article
C2 - 20599711
AN - SCOPUS:77955278967
VL - 398
SP - 420
EP - 425
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -