Expression of functional GPR35 in human iNKT cells

Silvia Fallarini, Laura Magliulo, Tiziana Paoletti, Claudia de Lalla, Grazia Lombardi

Research output: Contribution to journalArticle

Abstract

The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35; GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists [l-kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo [4,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from α-galactosylceramide (α-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins.In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release.

Original languageEnglish
Pages (from-to)420-425
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume398
Issue number3
DOIs
Publication statusPublished - Jul 2010

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Keywords

  • Cytokines
  • GPR35
  • Human iNKT cells
  • Kynurenic acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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