Expression of functional interleukin-3 receptors on Hodgkin and Reed-Sternberg cells

Donatella Aldinucci, Dalisa Poletto, Annunziata Gloghini, Paola Nanni, Massimo Degan, Tiziana Perin, Paola Ceolin, Francesca Maria Rossi, Valter Gattei, Antonino Carbone, Antonio Pinto

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Abstract

The human interleukin-3 receptor (IL-3R) is a heterodimeric complex consisting of an IL-3-specific α chain (IL-3Rα) and a common β chain (βc), this latter shared with the receptors for granulocyte-macrophage colony-stimulating factor and IL-5. Despite extensive research on cytokine circuitries regulating proliferation and survival of tumor cells in Hodgkin's disease (HD) the functional expression of IL-3Rs in this pathobiological entity has not yet been investigated. In the present study, we demonstrate that the great majority (>90%) of malignant Hodgkin and Reed-Sternberg cells of classic RD (19 of 19 analyzed cases) express IL-3Rα by immunostaining of frozen sections and cell suspensions from involved lymph nodes. Accordingly, HD cell lines (L428, KMH2, HDLM2, L1236) expressed the α and β chains of IL-3R both at the mRNA and protein level, with a molecular size of IL-3Rα identical (70 kd) to that expressed by human myeloid cells. Exogenous IL-3 promoted the growth of cultured Hodgkin and Reed-Sternberg cells, such effect being potentiated by IL-9 co-stimulation, and was able to partially rescue tumor cells from apoptosis induced by serum deprivation. This data suggests an involvement of IL-3/IL-3R interactions in the cellular growth of HD through paracrine mechanisms.

Original languageEnglish
Pages (from-to)585-596
Number of pages12
JournalAmerican Journal of Pathology
Volume160
Issue number2
Publication statusPublished - 2002

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Interleukin-3 Receptors
Reed-Sternberg Cells
Hodgkin Disease
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Interleukin-9
Interleukin-5
Frozen Sections
Myeloid Cells
Growth
Neoplasms
Cell Survival
Suspensions
Lymph Nodes
Apoptosis
Cytokines
Cell Line
Messenger RNA
Serum
Research

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of functional interleukin-3 receptors on Hodgkin and Reed-Sternberg cells. / Aldinucci, Donatella; Poletto, Dalisa; Gloghini, Annunziata; Nanni, Paola; Degan, Massimo; Perin, Tiziana; Ceolin, Paola; Rossi, Francesca Maria; Gattei, Valter; Carbone, Antonino; Pinto, Antonio.

In: American Journal of Pathology, Vol. 160, No. 2, 2002, p. 585-596.

Research output: Contribution to journalArticle

Aldinucci, Donatella ; Poletto, Dalisa ; Gloghini, Annunziata ; Nanni, Paola ; Degan, Massimo ; Perin, Tiziana ; Ceolin, Paola ; Rossi, Francesca Maria ; Gattei, Valter ; Carbone, Antonino ; Pinto, Antonio. / Expression of functional interleukin-3 receptors on Hodgkin and Reed-Sternberg cells. In: American Journal of Pathology. 2002 ; Vol. 160, No. 2. pp. 585-596.
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