Abstract
Cultured human melanocytes express mGlu5 metabotropic glutamate (mGlu) receptors, as shown by RT-PCR, immunocytochemistry, Western blot analysis, and measurement of agonist-stimulated polyphosphoinositide hydrolysis. The mGlu5 receptor agonists (S)-3,5-dihydroxyphenylglycine and quisqualate increased [3H-methyl]thymidine incorporation and melanocyte proliferation in subconfluent cultures, but impaired cell viability in confluent cultures. Both effects were prevented by 2-methyl-6-(2-phenyl-1-ethynyl)-pyridine, a potent and highly selective mGlu5 receptor antagonist. Agonists of other mGlu receptor subtypes (such as the mGlu2/3 receptor agonist, 2S,2'R,3'R-2-2',3'- dicarboxycyclopropylglycine, or the mGlu4/6/7/8 receptor agonist, L-2-amino- 4-phosphonobutanoate) or selective agonists of ionotropic glutamate receptors (N-methyl-D-aspartate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate, and kainate) did not affect melanocyte proliferation or viability. The presence of a receptor for glutamate, the major excitatory neurotransmitter, in human melanocytes is intriguing, mGlu5 receptors may be involved in the control of melanocyte proliferation (and perhaps in other functions), but harbor a potential toxicity and may therefore contribute to cell damage under pathological conditions. (C) 2000 Wiley-Liss, Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 364-372 |
| Number of pages | 9 |
| Journal | Journal of Cellular Physiology |
| Volume | 183 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2000 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
Expression of functional mGlu5 metabotropic glutamate receptors in human melanocytes. / Frati, C.; Marchese, C.; Fisichella, G.; Copani, A.; Nasca, M. R.; Storto, M.; Nicoletti, F.
In: Journal of Cellular Physiology, Vol. 183, No. 3, 2000, p. 364-372.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of functional mGlu5 metabotropic glutamate receptors in human melanocytes
AU - Frati, C.
AU - Marchese, C.
AU - Fisichella, G.
AU - Copani, A.
AU - Nasca, M. R.
AU - Storto, M.
AU - Nicoletti, F.
PY - 2000
Y1 - 2000
N2 - Cultured human melanocytes express mGlu5 metabotropic glutamate (mGlu) receptors, as shown by RT-PCR, immunocytochemistry, Western blot analysis, and measurement of agonist-stimulated polyphosphoinositide hydrolysis. The mGlu5 receptor agonists (S)-3,5-dihydroxyphenylglycine and quisqualate increased [3H-methyl]thymidine incorporation and melanocyte proliferation in subconfluent cultures, but impaired cell viability in confluent cultures. Both effects were prevented by 2-methyl-6-(2-phenyl-1-ethynyl)-pyridine, a potent and highly selective mGlu5 receptor antagonist. Agonists of other mGlu receptor subtypes (such as the mGlu2/3 receptor agonist, 2S,2'R,3'R-2-2',3'- dicarboxycyclopropylglycine, or the mGlu4/6/7/8 receptor agonist, L-2-amino- 4-phosphonobutanoate) or selective agonists of ionotropic glutamate receptors (N-methyl-D-aspartate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate, and kainate) did not affect melanocyte proliferation or viability. The presence of a receptor for glutamate, the major excitatory neurotransmitter, in human melanocytes is intriguing, mGlu5 receptors may be involved in the control of melanocyte proliferation (and perhaps in other functions), but harbor a potential toxicity and may therefore contribute to cell damage under pathological conditions. (C) 2000 Wiley-Liss, Inc.
AB - Cultured human melanocytes express mGlu5 metabotropic glutamate (mGlu) receptors, as shown by RT-PCR, immunocytochemistry, Western blot analysis, and measurement of agonist-stimulated polyphosphoinositide hydrolysis. The mGlu5 receptor agonists (S)-3,5-dihydroxyphenylglycine and quisqualate increased [3H-methyl]thymidine incorporation and melanocyte proliferation in subconfluent cultures, but impaired cell viability in confluent cultures. Both effects were prevented by 2-methyl-6-(2-phenyl-1-ethynyl)-pyridine, a potent and highly selective mGlu5 receptor antagonist. Agonists of other mGlu receptor subtypes (such as the mGlu2/3 receptor agonist, 2S,2'R,3'R-2-2',3'- dicarboxycyclopropylglycine, or the mGlu4/6/7/8 receptor agonist, L-2-amino- 4-phosphonobutanoate) or selective agonists of ionotropic glutamate receptors (N-methyl-D-aspartate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate, and kainate) did not affect melanocyte proliferation or viability. The presence of a receptor for glutamate, the major excitatory neurotransmitter, in human melanocytes is intriguing, mGlu5 receptors may be involved in the control of melanocyte proliferation (and perhaps in other functions), but harbor a potential toxicity and may therefore contribute to cell damage under pathological conditions. (C) 2000 Wiley-Liss, Inc.
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U2 - 10.1002/(SICI)1097-4652(200006)183:3<364::AID-JCP9>3.0.CO;2-X
DO - 10.1002/(SICI)1097-4652(200006)183:3<364::AID-JCP9>3.0.CO;2-X
M3 - Article
C2 - 10797311
AN - SCOPUS:0034057273
VL - 183
SP - 364
EP - 372
JO - Journal of cellular and comparative physiology
JF - Journal of cellular and comparative physiology
SN - 0021-9541
IS - 3
ER -