Expression of GABAA receptor subunits in rat brainstem auditory pathways: Cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus

M. L. Campos, C. De Cabo, W. Wisden, J. M. Juiz, D. Merlo

Research output: Contribution to journalArticle

Abstract

Inhibition by GABA is important for auditory processing, but any adaptations of the ionotropic type A receptors are unknown. Here we describe, using in situ hybridization, the subunit expression patterns of GABAA receptors in the rat cochlear nucleus, superior olivary complex, and dorsal and ventral nuclei of the lateral lemniscus. All neurons express the β3 and γ2L subunit messenger RNAs, but use different α subunits. In the dorsal cochlear nucleus, fusiform (pyramidal) and giant cells express α1, α3, β3 and γ2L. Dorsal cochlear nucleus interneurons, particularly vertical or tuberculoventral cells and cartwheel cells, express α3, β3 and γ2L. In the ventral cochlear nucleus, octopus cells express α1, β3, γ2L and δ. Spherical cells express α1, α3, α5, β3 and γ2L. In the superior olivary complex, the expression profile is α3, α5, β3 and γ2L. Both dorsal and ventral cochlear nucleus granule cells express α1, α6, β3 and γ2L; unlike their cerebellar granule cell counterparts, they do not express β2, γ2S or the δ subunit genes. The δ subunit's absence from cochlear nucleus granule cells may mean that tonic inhibition mediated by extrasynaptic GABAA receptors is less important for this cell type. In both the dorsal and ventral nuclei of the lateral lemniscus, α1, β3 and γ2L are the main subunit messenger RNAs; the ventral nucleus also expresses the δ subunit. We have mapped, using in situ hybridization, the subunit expression patterns of the GABAA receptor in the auditory brainstem nuclei. In contrast to many brain regions, the β2 subunit gene and γ2S splice forms are not highly expressed in auditory brainstem nuclei. GABAA receptors containing β3 and γ2L may be particularly well suited to auditory processing, possibly because of the unique phosphorylation profile of this subunit combination.

Original languageEnglish
Pages (from-to)625-638
Number of pages14
JournalNeuroscience
Volume102
Issue number3
DOIs
Publication statusPublished - Feb 5 2001

Fingerprint

Auditory Pathways
Cochlear Nucleus
GABA-A Receptors
Brain Stem
In Situ Hybridization
Octopodiformes
Messenger RNA
Superior Olivary Complex
Pontine Tegmentum
Pyramidal Cells
Interneurons
Giant Cells
gamma-Aminobutyric Acid
Genes
Phosphorylation
Neurons
Brain

Keywords

  • Hearing
  • In situ hybridization
  • Tonic inhibition

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of GABAA receptor subunits in rat brainstem auditory pathways : Cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus. / Campos, M. L.; De Cabo, C.; Wisden, W.; Juiz, J. M.; Merlo, D.

In: Neuroscience, Vol. 102, No. 3, 05.02.2001, p. 625-638.

Research output: Contribution to journalArticle

@article{49ae43683ae04c149bffe085f7570e56,
title = "Expression of GABAA receptor subunits in rat brainstem auditory pathways: Cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus",
abstract = "Inhibition by GABA is important for auditory processing, but any adaptations of the ionotropic type A receptors are unknown. Here we describe, using in situ hybridization, the subunit expression patterns of GABAA receptors in the rat cochlear nucleus, superior olivary complex, and dorsal and ventral nuclei of the lateral lemniscus. All neurons express the β3 and γ2L subunit messenger RNAs, but use different α subunits. In the dorsal cochlear nucleus, fusiform (pyramidal) and giant cells express α1, α3, β3 and γ2L. Dorsal cochlear nucleus interneurons, particularly vertical or tuberculoventral cells and cartwheel cells, express α3, β3 and γ2L. In the ventral cochlear nucleus, octopus cells express α1, β3, γ2L and δ. Spherical cells express α1, α3, α5, β3 and γ2L. In the superior olivary complex, the expression profile is α3, α5, β3 and γ2L. Both dorsal and ventral cochlear nucleus granule cells express α1, α6, β3 and γ2L; unlike their cerebellar granule cell counterparts, they do not express β2, γ2S or the δ subunit genes. The δ subunit's absence from cochlear nucleus granule cells may mean that tonic inhibition mediated by extrasynaptic GABAA receptors is less important for this cell type. In both the dorsal and ventral nuclei of the lateral lemniscus, α1, β3 and γ2L are the main subunit messenger RNAs; the ventral nucleus also expresses the δ subunit. We have mapped, using in situ hybridization, the subunit expression patterns of the GABAA receptor in the auditory brainstem nuclei. In contrast to many brain regions, the β2 subunit gene and γ2S splice forms are not highly expressed in auditory brainstem nuclei. GABAA receptors containing β3 and γ2L may be particularly well suited to auditory processing, possibly because of the unique phosphorylation profile of this subunit combination.",
keywords = "Hearing, In situ hybridization, Tonic inhibition",
author = "Campos, {M. L.} and {De Cabo}, C. and W. Wisden and Juiz, {J. M.} and D. Merlo",
year = "2001",
month = "2",
day = "5",
doi = "10.1016/S0306-4522(00)00525-X",
language = "English",
volume = "102",
pages = "625--638",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Expression of GABAA receptor subunits in rat brainstem auditory pathways

T2 - Cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus

AU - Campos, M. L.

AU - De Cabo, C.

AU - Wisden, W.

AU - Juiz, J. M.

AU - Merlo, D.

PY - 2001/2/5

Y1 - 2001/2/5

N2 - Inhibition by GABA is important for auditory processing, but any adaptations of the ionotropic type A receptors are unknown. Here we describe, using in situ hybridization, the subunit expression patterns of GABAA receptors in the rat cochlear nucleus, superior olivary complex, and dorsal and ventral nuclei of the lateral lemniscus. All neurons express the β3 and γ2L subunit messenger RNAs, but use different α subunits. In the dorsal cochlear nucleus, fusiform (pyramidal) and giant cells express α1, α3, β3 and γ2L. Dorsal cochlear nucleus interneurons, particularly vertical or tuberculoventral cells and cartwheel cells, express α3, β3 and γ2L. In the ventral cochlear nucleus, octopus cells express α1, β3, γ2L and δ. Spherical cells express α1, α3, α5, β3 and γ2L. In the superior olivary complex, the expression profile is α3, α5, β3 and γ2L. Both dorsal and ventral cochlear nucleus granule cells express α1, α6, β3 and γ2L; unlike their cerebellar granule cell counterparts, they do not express β2, γ2S or the δ subunit genes. The δ subunit's absence from cochlear nucleus granule cells may mean that tonic inhibition mediated by extrasynaptic GABAA receptors is less important for this cell type. In both the dorsal and ventral nuclei of the lateral lemniscus, α1, β3 and γ2L are the main subunit messenger RNAs; the ventral nucleus also expresses the δ subunit. We have mapped, using in situ hybridization, the subunit expression patterns of the GABAA receptor in the auditory brainstem nuclei. In contrast to many brain regions, the β2 subunit gene and γ2S splice forms are not highly expressed in auditory brainstem nuclei. GABAA receptors containing β3 and γ2L may be particularly well suited to auditory processing, possibly because of the unique phosphorylation profile of this subunit combination.

AB - Inhibition by GABA is important for auditory processing, but any adaptations of the ionotropic type A receptors are unknown. Here we describe, using in situ hybridization, the subunit expression patterns of GABAA receptors in the rat cochlear nucleus, superior olivary complex, and dorsal and ventral nuclei of the lateral lemniscus. All neurons express the β3 and γ2L subunit messenger RNAs, but use different α subunits. In the dorsal cochlear nucleus, fusiform (pyramidal) and giant cells express α1, α3, β3 and γ2L. Dorsal cochlear nucleus interneurons, particularly vertical or tuberculoventral cells and cartwheel cells, express α3, β3 and γ2L. In the ventral cochlear nucleus, octopus cells express α1, β3, γ2L and δ. Spherical cells express α1, α3, α5, β3 and γ2L. In the superior olivary complex, the expression profile is α3, α5, β3 and γ2L. Both dorsal and ventral cochlear nucleus granule cells express α1, α6, β3 and γ2L; unlike their cerebellar granule cell counterparts, they do not express β2, γ2S or the δ subunit genes. The δ subunit's absence from cochlear nucleus granule cells may mean that tonic inhibition mediated by extrasynaptic GABAA receptors is less important for this cell type. In both the dorsal and ventral nuclei of the lateral lemniscus, α1, β3 and γ2L are the main subunit messenger RNAs; the ventral nucleus also expresses the δ subunit. We have mapped, using in situ hybridization, the subunit expression patterns of the GABAA receptor in the auditory brainstem nuclei. In contrast to many brain regions, the β2 subunit gene and γ2S splice forms are not highly expressed in auditory brainstem nuclei. GABAA receptors containing β3 and γ2L may be particularly well suited to auditory processing, possibly because of the unique phosphorylation profile of this subunit combination.

KW - Hearing

KW - In situ hybridization

KW - Tonic inhibition

UR - http://www.scopus.com/inward/record.url?scp=0035808852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035808852&partnerID=8YFLogxK

U2 - 10.1016/S0306-4522(00)00525-X

DO - 10.1016/S0306-4522(00)00525-X

M3 - Article

C2 - 11226699

AN - SCOPUS:0035808852

VL - 102

SP - 625

EP - 638

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 3

ER -