Expression of glycosylated recombinant human myelin-associated glycoprotein on a neuroblastoma cell line and its reactivity with HNK-1 but not human anti-MAG antibodies

G. Spagnol, P. Doneda, B. Cavanna, M. Sgarzi, E. Manfredini, S. Allaria, G. Scarlato, E. Nobile-Orazio

Research output: Contribution to journalArticle

Abstract

A correctly glycosylated myelin-associated glycoprotein (MAG) must express the carbohydrate epitope HNK-1, which is the target antigen for IgM antibodies in some patients with neuropathy. We transfected a human MAG cDNA clone into the neuroblastoma cell line SK-N-SH and verified by immunoblot the expression of the HNK-1 epitope on the recombinant molecule. By the same method and by indirect immunofluorescence we did not find any reactivity of human anti-MAG IgM antibodies with glycosylated recombinant MAG and transfected neuroblastoma cells. These findings suggest that the mere presence of the HNK-1 epitope is probably not sufficient for MAG to be recognized by human antibodies and that other factors such as the concentration or fine structure of this epitope in MAG, which mostly depend on the cellular context, may be also critical for this reactivity.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalNeuroscience Letters
Volume246
Issue number3
DOIs
Publication statusPublished - Apr 10 1998

Fingerprint

Myelin-Associated Glycoprotein
Neuroblastoma
Epitopes
Cell Line
Antibodies
Immunoglobulin M
Indirect Fluorescent Antibody Technique
Complementary DNA
Clone Cells
Carbohydrates
Antigens
human MAG protein

Keywords

  • Anti-myelin-associated- glycoprotein antibodies
  • HNK-1
  • Myelin-assoclated glycoprotein
  • SK-N-SH

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of glycosylated recombinant human myelin-associated glycoprotein on a neuroblastoma cell line and its reactivity with HNK-1 but not human anti-MAG antibodies. / Spagnol, G.; Doneda, P.; Cavanna, B.; Sgarzi, M.; Manfredini, E.; Allaria, S.; Scarlato, G.; Nobile-Orazio, E.

In: Neuroscience Letters, Vol. 246, No. 3, 10.04.1998, p. 157-160.

Research output: Contribution to journalArticle

Spagnol, G. ; Doneda, P. ; Cavanna, B. ; Sgarzi, M. ; Manfredini, E. ; Allaria, S. ; Scarlato, G. ; Nobile-Orazio, E. / Expression of glycosylated recombinant human myelin-associated glycoprotein on a neuroblastoma cell line and its reactivity with HNK-1 but not human anti-MAG antibodies. In: Neuroscience Letters. 1998 ; Vol. 246, No. 3. pp. 157-160.
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AU - Cavanna, B.

AU - Sgarzi, M.

AU - Manfredini, E.

AU - Allaria, S.

AU - Scarlato, G.

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