TY - JOUR
T1 - Expression of HLA class II determinants by normal and chronic myeloid leukemia progenitors
AU - Piacibello, Wanda
AU - Aglietta, Massimo
AU - Stacchini, Alessandra
AU - Spinelli, Patrizia
AU - Salvetti, Laura
AU - Kerim, Simonetta
AU - Malavasi, Fabio
AU - Infelise, Vittorio
AU - Resegotti, Luigi
AU - Gavosto, Felice
PY - 1987
Y1 - 1987
N2 - It has been suggested that the expression of some HLA class II antigens, derived from three loci (DR, DP, DQ) is important in the regulation of both the immune response and the response of haemopoietic progenitors to regulation factors, such as acidic isoferritins (AIF), as well as in the interaction between T lymphocytes and erythroid progenitors (BFU-E). Changes in the expression of class II antigens have been reported on the surface of granulo-monocyte progenitors in chronic myeloid leukemia (CML) and correlated to the abnormal proliferation of such cells. In this study, monoclonal antibodies against DR and DQ monomorphic determinants were used to investigate the expression of these antigens on the surface of normal and CML bone marrow and peripheral blood BFU-E by means of complement mediated cytotoxicity. It was found that most normal and leukemic BFU-E express DR antigens. Antigen density tends to be greater on marrow as opposed to peripheral precursors. In addition, leukemic BFU-E are more sensitive to cytolytic treatment than their normal counterparts. Normal BFU-E do not express detectable amounts of DQ antigens, whereas these are present on a proportion of leukemic BFU-E.
AB - It has been suggested that the expression of some HLA class II antigens, derived from three loci (DR, DP, DQ) is important in the regulation of both the immune response and the response of haemopoietic progenitors to regulation factors, such as acidic isoferritins (AIF), as well as in the interaction between T lymphocytes and erythroid progenitors (BFU-E). Changes in the expression of class II antigens have been reported on the surface of granulo-monocyte progenitors in chronic myeloid leukemia (CML) and correlated to the abnormal proliferation of such cells. In this study, monoclonal antibodies against DR and DQ monomorphic determinants were used to investigate the expression of these antigens on the surface of normal and CML bone marrow and peripheral blood BFU-E by means of complement mediated cytotoxicity. It was found that most normal and leukemic BFU-E express DR antigens. Antigen density tends to be greater on marrow as opposed to peripheral precursors. In addition, leukemic BFU-E are more sensitive to cytolytic treatment than their normal counterparts. Normal BFU-E do not express detectable amounts of DQ antigens, whereas these are present on a proportion of leukemic BFU-E.
KW - chronic myeloid leukemia
KW - erythropoiesis
KW - HLA class II antigens
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U2 - 10.1016/0145-2126(87)90053-1
DO - 10.1016/0145-2126(87)90053-1
M3 - Article
C2 - 3470577
AN - SCOPUS:0023100191
VL - 11
SP - 285
EP - 290
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 3
ER -