Expression of housekeeping genes in Hodgkin's disease lymph nodes

Research output: Contribution to journalArticle

Abstract

Housekeeping genes, particularly actin, tubulin, and the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are widely used to estimate the amount and integrity of RNA in Northern blotting. In this work, the most reliable housekeeping gene for gene expression analysis of Hodgkin's disease (HD) lymph nodes was determined by comparing the conventional housekeeping genes, β-actin, β-tubulin, GAPDH, and the mouse gene LLRep3, that had been used previously in gene expression studies. It was found that the amounts of mRNA in these genes are very heterogeneous in HD lymph nodes. In contrast, their expression was relatively constant in tonsils undergoing a chronic inflammatory process. It is concluded that none of the housekeeping genes tested is suitable for the fine quantitative analysis of gene expression in HD lymph nodes.

Original languageEnglish
Pages (from-to)1110-1112
Number of pages3
JournalLeukemia
Volume5
Issue number12
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

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