Expression of HSP 70, immediate-early response and heme oxygenase genes in ischemic-reperfused rat liver

L. Tacchini, L. Schiaffonati, C. Pappalardo, S. Gatti, A. Bernelli-Zazzera

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Reperfusion of the liver after non-necrogenic ischemia induces the expression of the HSP gene and the synthesis of the hsp 70 protein, the best known among stress (heat-shock) proteins. EXPERIMENTAL DESIGN: We have studied the time course of the induction and the effects of cycloheximide treatment on the expression of c-fos, c-jun and the heat-shock gene HSP 70 in ischemic-reperfused livers; extracts of these livers have also been examined for the binding to a synthetic oligonucleotide containing the heat-shock consensus sequence (HSE) in order to reveal the possible presence of an active heat-shock factor (HSF) in ischemic-reperfused tissue. RESULTS: Expression of HSP 70 gene appears only after a certain threshold of cell damage, is preceded by induction of c-fos and c-jun but does not depend on ongoing protein synthesis. The binding of HSF to HSE seems to start during the late period of ischemia, although the subsequent reperfusion increases the effect. The level of heme-oxygenase mRNA, an indicator of oxidative stress, increases in the liver after reperfusion but the oxidative stress caused by CoCl2 treatment does not induce the expression of HSP 70 gene under the conditions of the present experiments. CONCLUSIONS: We suggest that, similar to heat-shock, protein malfolding occurring during ischemia may trigger the HSP 70 gene induction, which is then amplified by the subsequent reperfusion stress. A model of chemically induced oxidative stress seems to be unable to induce the HSP 70 gene expression with the same characteristics of heat shock or ischemia-reperfusion.

Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalLaboratory Investigation
Volume68
Issue number4
Publication statusPublished - 1993

Keywords

  • Gene expression
  • Heat-shock genes
  • Ischemia
  • Stress response

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Tacchini, L., Schiaffonati, L., Pappalardo, C., Gatti, S., & Bernelli-Zazzera, A. (1993). Expression of HSP 70, immediate-early response and heme oxygenase genes in ischemic-reperfused rat liver. Laboratory Investigation, 68(4), 465-471.